黄花矶松组织培养及培养基筛选研究

Research on Tissue Culture and Medium of Limoniumau-reum

试验选用种子培养的黄花矶松无菌苗的子叶、茎段、下胚轴作为外植体材料,研究不同外植体的离体培养技术及其适宜的培养基。结果表明,生长素2,4-D对不定芽诱导具有明显的促进作用,在其浓度为1.5 mg/L时诱导率最高,子叶是诱导不定芽的良好外植体,最适培养基为MS+2,4-D 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.0 mg/L。黄花矶松的最适增殖培养基为MS+6-BA 1.0 mg/L+NAA 1.0 mg/L,而且是以丛生芽的方式进行增殖的;最适生根培养基是1/2 MS+KT 1.0 mg/L+IBA1.0 mg/L。

Experiment chose seed development Limoniumau- reum no vaccine hypocotyl cotyledon stemsection as explant materials, study the culture of different explant technique, the results showed that： thesuitable medium auxin 2,4- D on adventitious bud induction had an obvious role in promoting, when itsconcentration was 1.5 mg/L induction rate was highest, cotyledon was good explant of adventitious budinduction, the optimal culture medium for MS＋2,4-D 1.5 mg/L＋6-BA 2.0 mg/L＋NAA 1.0 mg/L. Limoniumau-reum optimal proliferation medium was MS＋6-BA 1.0 mg/L＋ NAA 1.0 mg/L, and took the form of multipleshoot clumps proliferation, The optimum rooting medium was 1/2 MS＋ KT 1.0 mg/L＋ IBA 1.0 mg/L.