摘要
为了提高常春藤皂苷检测的灵敏度,以中华常春藤为试验材料,采用HPLC-ELSD对中华常春藤中常春藤苷C和α-长春藤皂苷的分析方法进行了研究。采用的色谱条件为：Welchrom C18（250 mm×4.6 mm,5μm）色谱柱;水-乙腈溶液为流动相,梯度洗脱;检测器为蒸发光散射检测器;流速1.0 mL/min;柱温30℃。常春藤苷C的进样量在0.19188-2.3985μg范围内峰面积的对数与进样量的对数之间线性关系良好;α-常春藤皂苷的进样量在0.15615-4.6844μg范围内峰面积的对数与进样量的对数之间线性关系良好。该方法灵敏度高,具有良好的稳定性和准确度。
In order to improve the detection sensitivity of saponins, a kind of high performance liquid chromatography（HPLC） coupled with evaporative light scattering detector（ELSD） was established for the determination of hederacoside C and α-hederin in Hedera nepalensis vat. sinensis. A welchrom column （250 mm×4.6 mm, 5 μm） was used with the mobile phase of water and acetonitrile solution. The flow rate was at 1.0 mL/min. This method showed good linearity for both hederacoside C （ranging from 0.19188 to 2.3985 μg） and α-hederin （ranging from 0.15615 to 4.6844 μg）. The results demonstrated that this method was precise, accurate and can be used for the determination of hederacoside C and α-hederin.
出处
《中国农学通报》
CSCD
2014年第13期190-193,共4页
Chinese Agricultural Science Bulletin
基金
作物种质创新与资源利用重点实验室科学基金"茶树中甲基化EGCG相关基因的全长cDNA克隆与功能研究"(12KFXM12)
