培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响

Effect of Inorganic Salts Concentrations in Medium and Culture Methods on Proliferation and Growth of Gardenia jasminoides Ellis

以栀子(Gardenia jasminoides Ellis)组培苗作为试验材料,研究培养基中无机盐浓度及培养方式对栀子组培苗增殖的影响,为栀子的大规模生产和快速繁殖提供理论依据。试验结果表明:MS培养基适合栀子的增殖培养;栀子增殖培养时总氮浓度以60 mmol/L为宜;NO3-/NH4+为40 mmol/L/20 mmol/L有利于栀子不定苗的增殖;反应器间歇浸没培养为最适宜的培养方式。

The study was conducted to investigate the effect of inorganic salts concentrations and culturemethods on the proliferation of Gardenia jasminoides, in order to supply theoretical bases for large-scale andrapid production. Gardenia jasminoides plantlets in vitro were used as experiment materials. The resultsshowed that the standard MS strength（1.0 MS） was optimal. In addition, 60 mmol/L nitrogen and 40 mmol/LNO3-mixed 20 mmol/L NH4＋in medium were the most appropriate for proliferation of Gardenia jasminoidesplantlets in vitro. Ebb and flood bioreactor culture was the most suitable culture method.