3种生物测定方法对香蕉花蓟马毒力测定的影响

Effect of Three Bioassay Methods on Toxicity of Insecticides Against Larvae of Thrips hawaiiensis

为探究杀虫剂对香蕉花蓟马科学合理的生物测定方法,采用离心管药膜法、浸叶法和叶管药膜法测定了5种常用杀虫剂原药对香蕉花蓟马2龄若虫的毒力。结果显示:3种生物测定方法对测定结果产生不同的影响,以叶管药膜法的毒力最大,其对5种药剂的毒力(LC50)顺序为:甲维盐(0.36 mg/L)>毒死蜱(0.90 mg/L)>吡虫啉(1.64 mg/L)>高效氯氰菊酯(4.70 mg/L)>噻嗪酮(12.53 mg/L),这与离心管药膜法在5种药剂的毒力顺序一致:甲维盐(1.63 mg/L)>毒死蜱(2.18 mg/L)>吡虫啉(6.59 mg/L)>高效氯氰菊酯(7.38 mg/L)>噻嗪酮(22.06 mg/L);采用浸叶法对5种药剂的毒力(LC50)顺序为:吡虫啉(2.15 mg/L)>甲维盐(2.29 mg/L)>高效氯氰菊酯(7.21 mg/L)>毒死蜱(7.40 mg/L)>噻嗪酮(14.95 mg/L)。上述3种生物测定方法都可以建立并应用于香蕉花蓟马的生物测定,离心管药膜法更适合触杀毒力测定。

In an effort to test scientific and reasonable bioassay of Thrips hawaiiensis, laboratory studies wereconducted on the effect of three bioassay methods including centrifuge tube residual bioassay, leaf- dipbioassay and leaf- tube residual bioassay on toxicity of five original insecticides to against larvae of T.hawaiiensis. It was revealed that toxicity results of the 5 insecticides varied differently among the treatments,leaf-tube residual bioassay performed the highest toxicity and the toxicity（LC50） order was emamectin-benzoate（0.36 mg/L） chlorpyrifos（0.90 mg/L） imidacloprid（1.64 mg/L） betacy- permethrin（4.70 mg/L） buprofezin（12.53 mg/L）, which was consistent with centrifuge tube residual bioassay, for emamectin-benzoate（1.63 mg/L） chlorpyrifos（2.18 mg/L） imidacloprid（6.59 mg/L） betacy- permethrin（7.38 mg/L） buprofezin（22.06 mg/L）. Whereas toxicity order was imidacloprid（2.15 mg/L） emamectin- benzoate（2.29 mg/L） betacy-permethrin（7.21 mg/L） chlorpyrifos（7.40 mg/L）buprofezin（14.95 mg/L） when leaf-dip bioassay was applied in the research. In summary, present study suggested above three methods would beestablish and apply in laboratory bioassay on Thrips hawaiiensis, and centrifuge tube residual bioassay wasmore suitable for using in contact toxicity bioassay.