甘蔗与蔗茅杂交F_2BC_1代的染色体遗传分析

Chromosomal Transmission of F_2BC_1Progenies Between Sugarcane and Erianthus fulvus

为更有效的将蔗茅杂交后代应用于现代甘蔗新品种的选育及对旧有甘蔗品种进行改良提供科学依据,采用压片法制备甘蔗与蔗茅(2n=20)杂交的F2及F2BC1子代及其亲本材料的染色体玻片,对其杂交获得的F2及F2BC1世代材料进行染色体数目的传递分析,以探索其染色体传递机制。结果表明,各后代材料的体细胞染色体数目均不恒定;双亲染色体在F2代04/14中以"2n+n"方式进行传递,在F2BC1代中均以"n+n"的方式进行传递。研究结果可为这些材料在甘蔗育种中的进一步利用提供细胞学依据。

To effectively using the F2 and F2BC1 progenies of E. fulvus in sugarcane breeding for new sugarcane cuhivars selection or the old sugarcane cultivars modifieation, it is necessary to further study the chromosomal transmission of parents in the hybrids. The root-tips of different generation clones were collected, treated following the wall degradation hypotonic method, and prepared the slides used for chromosome counting of somatic cells. The results showed that, all of the chromosome numbers of hybrids were variable in different somatic cells, and chromosomes of parents were transmitted by ＂2n＋n＂ into the somatic cells of F2 hybrid and ＂n＋n＂ into F2BC1 hybrids. The results of current study can provide cytological information for the further use of these hybrids in sugarcane breeding programs.