摘要
为更有效的将蔗茅杂交后代应用于现代甘蔗新品种的选育及对旧有甘蔗品种进行改良提供科学依据,采用压片法制备甘蔗与蔗茅(2n=20)杂交的F2及F2BC1子代及其亲本材料的染色体玻片,对其杂交获得的F2及F2BC1世代材料进行染色体数目的传递分析,以探索其染色体传递机制。结果表明,各后代材料的体细胞染色体数目均不恒定;双亲染色体在F2代04/14中以"2n+n"方式进行传递,在F2BC1代中均以"n+n"的方式进行传递。研究结果可为这些材料在甘蔗育种中的进一步利用提供细胞学依据。
To effectively using the F2 and F2BC1 progenies of E. fulvus in sugarcane breeding for new sugarcane cuhivars selection or the old sugarcane cultivars modifieation, it is necessary to further study the chromosomal transmission of parents in the hybrids. The root-tips of different generation clones were collected, treated following the wall degradation hypotonic method, and prepared the slides used for chromosome counting of somatic cells. The results showed that, all of the chromosome numbers of hybrids were variable in different somatic cells, and chromosomes of parents were transmitted by "2n+n" into the somatic cells of F2 hybrid and "n+n" into F2BC1 hybrids. The results of current study can provide cytological information for the further use of these hybrids in sugarcane breeding programs.
出处
《中国农学通报》
CSCD
2014年第15期81-85,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"蔗茅杂种染色体传递规律及特异DNA序列定位的研究"(30960045)
云南省现代农业甘蔗产业技术体系建设项目"育种与繁育研究室岗位"(云财教[2013]160号)
云南省重点新产品开发计划项目"高产高糖宿根甘蔗新品种选育及产业化开发"(农业部分
2012BB014)
云南农业大学高原山地作物可持续生产系统研究省创新团队项目"高原山地特色作物种质创新及利用"(云科人发[2012]18号)
云南省教育厅基金项目"甘蔗野生种蔗茅的遗传多样性"(2011C174)
关键词
甘蔗
蔗茅
杂交后代
染色体传递
sugarcane
Erianthus fulvus
hybrid
chromosomal transmission