姜黄素通过抑制ERK1/2磷酸化减弱ET-1诱导的大鼠VSMCs增殖

Effect of Curcumin on Proliferation of Rat Vascular Smooth Muscle Cells Induced by Endothelin-1 and Expression of Phosphorylated ERK1 /2

目的观察姜黄素(curcumin)对内皮素-1(ET-1)诱导培养的离体大鼠血管平滑肌细胞(VSMCs)增殖及p44/42丝裂原活化蛋白激酶(p44/42 MAPK,ERK1/2)表达的影响。方法组织贴块法培养大鼠胸主动脉VSMCs。分别以ET-1 10-8mol/L,ET-1 10-8mol/L联合浓度为10-6mol/L、10-5mol/L、10-4mol/L的姜黄素作用于体外培养的VSMCs 24 h,采用细胞计数检测VSMCs的增殖,3H-胸腺密啶掺入(3H-TdR)法测定VSMCs的DNA合成,western bolt法检测VSMCs磷酸化ERK1/2的表达。结果与对照组比较,ET-1能显著刺激VSMCs增殖,姜黄素能显著抑制ET-1诱导的VSMCs增殖,且呈剂量依赖关系;ET-1能显著刺激VSMCs磷酸化ERK1/2的表达,该作用可被姜黄素所抑制。结论姜黄素能抑制ET-1刺激的VSMCs的增殖,其作用可能与抑制磷酸化ERK1/2的表达相关。

Objective To study effect of curcumin on proliferation of rat vascular smooth muscle cells（ VSMCs） in-duced by endothelin-1（ET-1） and expression of phosphorylated p44/42 mitogen-activated protein kinase （p44/42 MAPK, ERK1/2）. Methods Rat VSMCs cultured in vitro were exposed for 24 h to 10 -8 mol/L ET-1,10 -8 mol/L ET-1 plus 10 -6 mol/L,10 -5 mol/L,10 -4 mol/L curcumin repectively. The effect of curcumin on VSMCs growth and DNA synthesis were investigated by cell counting,3H-thymidine incorporation,the expression of phosphorylated ERK1/2（p-ERK1/2） were determined by western blotting. Results Compaered with control group,VSMCs exposed to ET-1 showed significantly en-hanced proliferation （P〈0. 05）,curcumin could inhibit proliferation of VSMCs induced by ET-1（P〈0. 05）,there are dose dependent inhibitory effects in different concentration of curcumin. ET-1 treatment also increased significantly the expression of p-ERK1/2,curcumin could decrease the effection （P〈0. 05）. Conclusion Curcumin can inhibit the proliferation of rat VSMCs and the expression of p-ERK1/2 induced by ET-1.