阿司匹林对小鼠胚胎腭裂发生的作用研究

The effect of aspirin on cleft palate incidence of mice embryo

目的：探讨阿司匹林对小鼠胚胎腭裂发生的作用，确立阿司匹林诱导小鼠腭裂模型的最佳剂量，为深入研究腭裂提供动物实验模型。方法分别将确定孕期的BALB/C雌鼠随机分为5组，即对照组和实验Ⅰ∽Ⅳ组。胚胎第10∽12天（embryo 10∽embryo 12，E10∽ E12）时，每日定时给予对照组孕鼠灌胃生理盐水，实验Ⅰ、Ⅱ、Ⅲ、Ⅳ组孕鼠分别灌胃阿司匹林180、210、240、270 mg/kg。于E18时处死孕鼠，称量并计算孕鼠和胚胎的体质量，记录胚胎腭裂数及发育情况。结果对照组腭裂发生率明显低于实验Ⅲ、Ⅳ组，差异有统计学意义（χ2＝15．057， P ＜0.01；χ2＝175．51，P ＜0.01）。实验Ⅲ、Ⅳ组比较，实验Ⅲ组腭裂发生率高于实验Ⅳ组，差异有统计学意义（χ2＝47．42，P ＜0.01）；各组间胚胎体质量的差别无统计学意义（P ＞0.05）。结论阿司匹林可致小鼠胚胎腭裂发生，240 mg/kg阿司匹林是建立BALB/C胎鼠腭裂模型的最佳剂量。

Objective To define the optimal dose of aspirin induced cleft palate in BALB/C mice and to pro-vide a reliable model of cleft palate for further study .Methods Pregnant mice at specific period were divid-ed into five groups randomly ：experimental groups of Ⅰ ,Ⅱ ,Ⅲ ,Ⅳand control group .The experimental groups were perfued with gastric 180 ,210 ,240 ,270 mg/kg BW ,while the control group only physiologi-cal saline respectively on embryo 10 to embryo 12 .Pregnant mice were killed on embryo 18 to weigh preg-nant mice and embryos and record the number of embryonic development and cleft palate .Results Weight of pregnant mice was significantly different （χ2 = 15 0.57 ,P 〈 0.01;χ2 = 17 5.51 ,P 〈0 .01） .Incidence of embryonic cleft palate had significant difference between control group and experimental Ⅲ ,Ⅳgroups ,the control group was lower than that in group experiment Ⅲ ,Ⅳ（χ2 =4 7.42 ,P 〈0.01） .The difference was statistically significant that the incidence of cleft palate in experimental group Ⅲ was higher than the ex-perimental group Ⅳ .There was no difference in embryonic weight among each group （P 〉0.05） .Conclu-sion 240 mg/kg is the optimal dose of aspirin to induce cleft palate in BALB/C mice .