摘要
目的 探讨膀胱癌组织中N-钙黏附素蛋白表达与临床病理特征及预后的关系.方法 采用免疫组化方法检测2003年1月至2012年4月145例膀胱尿路上皮癌组织及25例正常膀胱组织中N-钙黏附素蛋白的表达情况.145例膀胱尿路上皮癌患者中,男104例,女41例.年龄49~83岁,中位年龄70岁,其中≤65岁57例,>65岁88例.肿瘤大小≤3 cm 79例,>3 cm 66例.肿瘤单发86例,多发59例.病理分级G1级44例,G2~G3级101例;分期(AJCC2002标准)Ta~T1期94例,T2~T4期51例.分析N-钙黏附素蛋白表达与膀胱癌分级、分期、数目、大小、预后及患者年龄、性别的关系. 结果 145例膀胱癌组织中N-钙黏附素蛋白表达阳性率为54.2%(76/145),正常组织中N-钙黏附素蛋白表达阳性率为12.0%(3/25),两组间差异有统计学意义(P<0.01).G1级和G2~ G3级别膀胱癌中N-钙黏附素蛋白表达阳性率分别为38.6% (17/44)、58.4%(59/101),差异有统计学意义(P=0.028).非肌层浸润性癌和肌层浸润性癌组织中N-钙黏附素蛋白表达阳性率分别为45.7%(43/94)和64.7%(33/51),差异有统计学意义(P=0.029).N-钙黏附素蛋白表达与患者年龄、性别、肿瘤的大小及数目无明显相关性(P>0.05).肌层浸润性癌患者随访4~103个月,平均42.5个月,N-钙黏附素蛋白阳性表达患者总生存率24.2% (8/33),N-钙黏附素蛋白阴性表达患者总生存率66.7%(12/18),Kaplan-Meier生存分析显示,两组间总生存率差异有统计学意义(P=0.002 2).多因素Cox回归分析显示N-钙黏附素蛋白表达是膀胱癌预后的重要因素. 结论 N-钙黏附素蛋白表达在膀胱癌组织中明显高于正常组织,N-钙黏附素蛋白的检测有助于膀胱癌的诊断及预后评估.
Objective To explore the expression and significance of N-cadherin in urothelial bladder cancer and analyse its relation to clinicpathologic and prognosis of bladder cancer.Methods The expression of N-cadherin in 145 urothelial bladder cancer and 25 normal bladder tissues was detected by immunuhistochemisty,and correlations between N-cadherin and clinicopathologic features were analysed.Results The positive rate of N-cadherin protein was significantly higher in bladder cancer than in normal bladder tissue (P〈0.01).The positive rate of N-cadherin protein was 38.6% in G1 bladder cancer,58.4% in G2-G3 bladder cancer,and its difference was significant (P =0.028).The expression was significantly lower in non-muscle-invasive bladder cancer than in muscle invasive bladder cancer (45.7% vs 64.7%,P=0.029).The muscle-invasive bladder cancer patients were followed up 4-103 months.Among those,the overall survival with positive expression of N-cadherin protein was 24.2% (8/33),and the overall survival with negative expression of N-cadherin protein was 66.7 % (12/18).Kaplan-Meier analysis showed the positive expression of N-cadherin was significantly associated with overall survival of patients with muscle-invasive-bladder cancer (P=0.002 2).Multivariate Cox analysis showed that N-cadherin expression was an important prognostic factor.Conclusions The expression of N-cadherin protein was high in bladder cancer.The detection of the expression of N-cadherin protein is associated with the diagnosis and prognosis of bladder cancer.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2014年第5期337-340,共4页
Chinese Journal of Urology
基金
上海市科学技术委员会自然基金(12ZR1417700)
浦东新区卫生系统重点学科群
关键词
膀胱肿瘤
癌
钙黏着糖蛋白类
预后
Urinary bladder neoplasms
Carcinoma
Cadherins
Prognosis