外伤性脾破裂大出血失血回收对患者血浆自由基活性的影响

Effect of Intraperitoneal Blood Salvage Technique on Activities of Serum Free Radicals after Splenic Rupture Hemorrhage

目的研究外伤性脾破裂大出血失血回收对患者血浆自由基即超氧化物歧化酶（superoxide dismutase,SOD）和丙二醛（malondialdehyde,MDA）活性的影响。方法 154例外伤性脾破裂大出血病人按输自、异体血分为失血回收组（即自体组：124例）和输异体血组（即异体组：30例）。IBST组回收失血,经过滤、离心、分离和洗涤,回输患者。两组分别于术前、术后及术后1、3、5、7天检测血清SOD、MDA含量。结果在输血〉2000 ml组中,自体组在术后1、3、5、7天血清SOD明显高于、MDA明显低于异体组各对应时相点,且较快恢复正常。在〈1000 ml、1000~2000 ml这两组中自体组血浆SOD明显高于、MDA明显低于异体组各对应时相点。结论外伤性脾破裂大出血后采取失血回收,不会进一步加剧病人的氧自由基损害,相反可增强机体清除自由基的能力。

Objective To study the effect of intraperitoneal blood salvage technique（IBST） on activities of serum superoxide dismutase（SOD） and malondialdehyde（MDA） after splenic rupture hemorrhage. Methods A total of 154 patients after splenic rupture hemorrhage were divided into IBST group and homologous transfusion group（HOM group）. The serum levels of SOD and MDA in peripheral vein were detected at 6 timepoints. The curative effects of the IBST were investigated. Results The serum levels of SOD and MDA of 〉2000 ml〈 1000 ml or 1000- 2000 ml blood loss patients in IBST group was significantly different from that in the HOM group at 1,3,5 and 7 days after operation. SOD was significantly higher,and MDA was much lower in IBST group than in HOM group. Conclusion IBST can promote the activities of SOD and reduce the activities of MDA to improve the clearence of oxygen free radical levels in the patients suffering from splenic rupture nemorrhage.