GC-MS法比较水蒸气蒸馏法和超临界CO_2萃取法提取复方苦黄方挥发性成分

Comparison of Steam Distillation and Supercritical CO_2 Method in the Extraction of Volatile Constituents from Compound Kuhuang Prescription by GC-MS

比较水蒸气蒸馏（简称SD）法和超临界CO：萃取法（简称SFE—CO：萃取法）提取复方苦黄方中蛇床子、防风、苍术等三味中药的挥发性成分及相对含量。方法：采用气相色谱一质谱联用（GC—MS）法，对SD法提取的挥发油和SFE-CO，萃取法得到的萃取物进行分析，比较两种提取物中的化学成分，并用峰面积归一化法比较各成分的相对含量。结果：SD法提取的挥发油中分离鉴定出36种成分，SFE—CO：法得到的萃取物中分离鉴定出31种成分，其中22种成分相同，相对分子量主要集中在200～230之间。结论：SFE—CO：法对复方苦黄方中蛇床子、防风、苍术等三味中药有效成分的提取具有更高的选择性，是更适合本方剂的提取方法。

To compare the steam distillation（SD） and supercritical CO2 （SFE-CO2） method in the extraction of vola- tile constituents from such 3 traditional Chinese drugs as cnidium mormieri, radix sileris and atractylodes lancea in compound Kuhuang prescription. Methods： Essential oil was extracted from the 3 traditional Chinese drugs by SD and SFE-CO2 method, respectively. The components were identified by GC-MS, and their relative contents were calculated with peak area normalization method. Results： To- tally 36 components were isolated and identified using SD extraction and 31 components were identified using SFE-CO2 extraction, a- mong them, 22 components were the same and their relative molecular weight mainly concentrated within 200-230. Conclusion： SFE- CO2 method can extract effective components from the 3 traditional Chinese drugs in compound Kuhuang prescription with higher selec-tivity, which is the more suitable extraction method for essential oil from the prescription.