青蒿琥酯对人白血病K562,K562/ADM细胞凋亡的作用及对NF-κB p65表达的影响

Effects of Artesunate on K562,K562 /ADM Cell Apoptosis and NF-κB p65 Expression in Human Leukemia

目的:观察青蒿琥酯对人白血病K562、K562/ADM细胞凋亡以及对p65表达的影响。方法:采用流式细胞仪检测青蒿琥酯在不同浓度和不同时间段对K562、K562/ADM细胞凋亡的影响,采用Western blot法检测15μmol·L-1青蒿琥酯在不同时间对K562细胞NF-κB p65表达的影响。结果:青蒿琥酯对K562细胞凋亡影响不明显,但对阿霉素耐药K562/ADM细胞影响较大,青蒿琥浓度为7.5μmol·L-1和15μmol·L-1时,K562/ADM细胞的调亡率明显高于K562细胞(P<0.01),15μmol·L-1青蒿琥酯作用后4 h后,K562/ADM细胞的调亡率明显增加(P<0.01);经15μmol·L-1青蒿琥酯作用后,p65表达随时间的增加而明显降低(P<0.01)。结论:青蒿琥酯通过下调P65的表达而诱导白血病细胞凋亡。

To study the effects of artesunate on K562, K562/ADM cell apoptosis and NF-κB p65 expression in hu- man leukemia. Methods： The effect of artesunate with different concentrations on K562 and K562 /ADM cell apoptosis at various times was studied by a flow cytometer. The effect of artesunate with 15μmol·L^-1 on NF-κB P65 expression in K562 was explored using a Western blot method. Results： Artesunate showed little effect on K562 cell apoptosis, while exhibited notable effect on K562 / ADM cell apoptosis. K562/ADM cells were more sensitive than K562 to artesunate at the concentration of 7.5 μmol·L^-1and 15 μmol·L^-1（ P 〈 0.01）, and after 4-hour treatment with 15 μmol·L^-l artesunate, K562/ADM cell apoptosis was much higher than K562（P 〈 0.01 ）. Artesunate could reduce leukemia cell p65 expression at the concentration of 15μmol·L^-1. Conclusion： Artesu-nate can induce the apoptosis of leukemia cells by down-regulating the expression of p65.