HPLC法同时测定千里光中对羟基苯甲酸和邻羟基苯甲酸的总含量及对羟基苯乙酸的含量

Simultaneous Determination of Total Content of p-Hydroxybenzoic Acid plus o-Hydroxybenzoic Acid and pHydroxybenzeneacetic Acid in Senecio Scandens Buc

建立同时测定千里光药材中对羟基苯甲酸和邻羟基苯甲酸总含量及对羟基苯乙酸含量的方法。方法：采用HPLC法，色谱柱：Shiseido（Fine Chemicals）Capcell Pak C18柱（250mmx4.6mm，5μm）；流动相：甲醇：水：甲酸（13：87：0．5）；流速：1.0ml·min^-1；检测波长：240nm；柱温为室温。结果：对羟基苯甲酸和邻羟基苯甲酸的总浓度在0．025～0．400mg·ml^-1范围内线性关系良好（r=0．9998），平均加样回收率为97．59％，RSD=1．22％（n=6）；对羟基苯乙酸的浓度在0．05—0．80mg·ml6-1范围内线性关系良好（r：0．9998），平均加样回收率为98．07％，RSD=1．90％（n=6）。结论：该方法操作简单。重现性好，适用于千里光中对羟基苯甲酸、邻羟基苯甲酸和对羟基苯乙酸的含量测定。

To set up an HPLC method for the simultaneous determination of p-hydroxybenzoic a6id plus o-hydroxy- benzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch. Methods： The Column was a Shiseido （ Fine Chemicals） Cap-cell Pak Cls （250 mm×4.6 mm, 5μm） column at the room temperature. The mobile phase was methanol-water-formic acid （13：87： 0.5 ） at a flow rate of 1.0 ml·min^-1. The detection wavelength was 240nm. Results： p-Hydroxybenzoic acid plus o-hydroxybenzoic acid had a favorable linear relationship within the range of 0.025-0. 400 mg·ml-1, the regression equation was Y = 5.94×106X ＋ 2. 46×10^4 （ r = 0. 999 8 ） , the average recovery was 97.59% and RSD was 1.22%. p2Hydroxybenzeneaeetic acid had a favorable line- ar relationship within the range 0.05-0.80 mg·ml -!, the regression equation was Y = 4. 09 x 106X ＋ 1.12×10^4 （ r = 0.999 8 ）, the average recovery was 98.07% and RSD was 1.90%. Conclusion ： The method is simple, feasible and reproducible. It can he used in the quality control of p-hydroxybenzoic acid plus o-hydroxybenzoic acid and p-hydroxybenzeneacetic acid in Senecio scandens Buch.