正交设计优选乳宁丸中总氨基酸的测定工艺

Determination Optimization of Total Amino Acids in Runing Pills by Orthogonal Design

目的:优选出乳宁丸中总氨基酸的最佳测定工艺。方法:采用L9(34)正交试验设计,以水解温度、水解时间和盐酸浓度为考察因素,乳宁丸中总氨基酸的含量和得率为考察指标,确定乳宁丸中总氨基酸的最佳提取条件。结果:最佳测定工艺为水解温度110℃,水解时间为22 h,盐酸浓度为6 mol·L-1。结论:该优化测定工艺可用于乳宁丸中总氨基酸的测定,为乳宁丸的进一步研究提供了参考。

To optimize the determination technology for the total amino acids in Runing pills. Methods： The process was optimized by L9（ 3^4 ） orthogonal design using the hydrolysis temperature, hydrolysis time and hydrochloric acid concentration as the evaluation factors, and the content and yield of total amino acids as the evaluation indices. Results： The optimal determination condi- tions were as follows： the hydrolysis temperature was 110℃, the hydrolysis time was 22 h and the hydrochloric acid concentration was 6 mol·L^-1. Conclusion： The optimal determination technology can be applied in the total amino acids from Runing pills, which provides the basis for the further research of Runing pills.