摘要
本研究建立了饲料和鸡肉中利巴韦林的UPLC-MS/MS分析方法。样品经三氯乙酸-乙腈溶液提取，离心后提取液经PBA固相萃取小柱净化，0.1 mol/L甲酸水溶液溶解，进行UPLC-MS/MS分析。结果显示，利巴韦林在10.0～500.0 g/L浓度范围内线性关系良好，r2=0.9991。以浓缩饲料、配合饲料、预混料和鸡肉等为添加基质，其回收率在90.0%～114.5%，检出限为25.0 g/kg，定量限为50.0 g/kg。表明本方法灵敏度高、专属性好、操作简单、结果可靠，可满足饲料和鸡肉中利巴韦林的分析检测。
A method of Ultra Performance Liquid Chromatography-Tandem mass spectrometry（UPLC-MS/MS） for determination of ribavirin in feeds and chicken was developed. The drugs were extracted with acetonitrile/trichloroacetic acid from analyte, then cleaned up with PBA cartridges. The elu-ent was assayed by UPLC-MS/MS. The detection and quantification limits were 25.0 μg/kg and 50.0 μg/kg respectively. The average recoveries were 90.0%~114.5%. The real sample tests showed this meth-od can be used for the sensitive and accurate determination of ribavirin in feeds and chicken.
出处
《现代畜牧兽医》
2014年第5期15-19,共5页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
利巴韦林
饲料
鸡肉
UPLC-MS/MS
Ribavirin
Feeds
Chicken
UPLC/MS/MS
