劳力型与居民型热射病大鼠模型的比较

The comparison of exertional and classic heat stroke rat models

目的构建劳力型热射病大鼠模型,与居民型热射病大鼠模型比较。方法雄性SPF级SD大鼠随机分为劳力型热射病组(E-HS)、居民型热射病组(C-HS)和相应的正常对照组(E-NC和C-NC组),每组16只。热射病组大鼠予39℃热暴露,E-HS组除了给予39℃热暴露外,同时让其在跑台持续运动。监测各组大鼠核心体温和动脉收缩压变化,对比核心体温调节反应和生存时间。结果 E-HS和C-HS组大鼠表现出不同的核心体温调节特征。尽管最大核心体温差异无统计学意义,但是对比C-HS组大鼠,E-HS组大鼠核心体温上升速率显著加快(P<0.05),成模时间显著缩短(P<0.05),总热负荷和严重热负荷均显著减少(P<0.05),预后更差(P<0.05)。结论劳力型热射病大鼠模型相较与居民型热射病大鼠预后差,劳力型热射病大鼠模型的建立可为研究劳力型热射病的病理生理机制提供基础。

Objective To construct exertional and classic heat stroke rat models, and investigate the difference of heat stress response between these two models. Methods Male SD rats were randomly divided into exertional heat stroke （E- HS）, classic heat stroke （C-HS）, exertional normal control （E-NC） and classic normal control （C-NC） groups. Rats of E-HS and C-HS groups were kept in artificial climate chamber, and exposed to 39℃ heat stress,meanwhile,let rats of E-HS group continue exercise in the treadmill. Core temperature of rats were monitored by rectal thermal couple, and systolic blood pressure （SBP） of rats were monitored by non-invasive artery blood pressure monitory instrument. Heat stress response of E-HS and C-HS was quantitatively analyzed, and survival time was compared by K-M survival analysis. Results There was a significant difference in thermoregulatory profile between E-HS and C-HS rats. Although no difference in maximum core temperature has been observed between E-HS and C-HS rats, but in comparison with C- HS rats, E-HS rats had significant faster heating rate （P〈0.05）, shorter heat stress time period, and lighter total thermal load （P〈0.05） and severe thermal load （P〈0.05）. E-HS rats had significant poor survival than C-HS rats （P〈 0.05）. Conclusion We established the EHS rat model and the model is more adaptable for the investigation of experimental exertional heat stroke, and further investigation of this model may help to understand the mechanism of the pathogenesis of exertional heatstroke.