柚皮苷抗高糖诱导的心肌细胞损伤作用与抑制STAT3通路有关

Naringin Protects H9c2 Cardiac Cells Against High Glucose-induced Injury by Inhibiting STAT3 Pathway

目的探讨柚皮苷是否通过抑制信号转导子和转录激活子3(STAT3)通路保护H9c2心肌细胞对抗高糖引起的损伤。方法应用35 mmol/L的高浓度葡萄糖(高糖,HG)处理H9c2心肌细胞24 h,建立HG损伤心肌细胞模型;细胞计数试剂盒8测定细胞存活率;Hoechst 33258核染色荧光显微镜照相法测定细胞凋亡;双氯荧光素染色/荧光显微镜照相法测定胞内活性氧(ROS)水平;罗丹明123染色法测定线粒体膜电位(MMP);Western blot法测定STAT3蛋白表达水平。结果 HG处理H9c2心肌细胞24 h能引起明显的损伤,使细胞存活率降低,凋亡细胞数量和胞内ROS生成增多,MMP丢失;HG能增加STAT3磷酸化水平;柚皮苷预处理能明显抑制HG上调STAT3磷酸化水平这一作用;柚皮苷和STAT3通路抑制剂AG490能阻断HG对心肌细胞的上述损伤作用,包括细胞毒性、凋亡、ROS生成增多及MMP丢失等。结论柚皮苷可通过抑制STAT3通路保护H9c2心肌细胞对抗高糖引起的损伤。

Aim To explore whether naringin（ NRG） protects H9c2 cardiac cells against high glucose（ HG）induced injury by inhibition of signal transducers and activators of transcription 3（ STAT3） pathway. Methods H9c2 cardiac cells were treated with 35 mmol / L glucose（ HG） for 24 h to establish a model of HG-induced injury; Cell counter kit-8（ CCK-8） was used to measure cell viability; Apoptotic cells were tested by Hoechst 33258 nuclear staining followed by fluorescence imaging; Intracellular levels of reactive oxygen species（ ROS） were detected by 2＇,7＇-dichlorfluoresceindiacetate（ DCFH-DA） staining,followed by fluorescence imaging; Mitochondrial membrane potential（ MMP） was measured by a fluorescent dye,rhodamine 123（ Rh123）,followed by fluorescence imaging; The expression levels of STAT3 protein were detected by Western blot assay. Results Treatment of H9c2 cardiac cells with HG for 24 h significantly induced injuries,evidenced by a decrease in cell viability,increases in amount of apoptotic cells and intracellular ROS production,as well as a loss of MMP; HG enhanced the expression of phosphorylated（ p）-STAT3; Pretreatment with NRG markedly inhibited the up-regulation of expression of p-STAT3 induced by HG; Pretreatment with NRG or AG490,an inhibitor of STAT3 pathway,attenuated the above HG-induced injuries,including cytotoxicity,apoptosis,increase in ROS production and a loss of MMP,in H9c2 cardiac cells. Conclusion NRG may protect H9c2 cardiac cells against the HG-induced injuries by inhibiting the STAT3 pathway.