苦菊提取物对肝损伤的保护作用及其安全性致突变作用的研究

Protection of CichoriumendiviaL.Extract from Liver Injury and Its Mutagenesis

目的考察苦菊提取物对肝损伤的影响及其致突变作用。方法通过噻唑蓝(MTT)法和2,7-二氯二氢荧光素乙酰乙酸(DCFH-DA)荧光染色,检测苦菊提取物对2,2’-偶氮-双-(2-脒基丙烷)氯化二氢(AAPH)致HepG2细胞损伤的存活率以及胞内活性氧(ROS)水平的影响;建立四氯化碳(CCl4)致小鼠肝急性损伤模型,以观察苦菊提取物对急性肝损伤小鼠的肝组织病理变化和血清中丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)水平的影响;以鼠伤寒沙门氏菌回复突变(Ames)试验检测苦菊提取物的致突变作用。结果 AAPH处理HepG2细胞后,细胞存活率下降,胞内ROS水平上升,而加入不同浓度的苦菊提取物则可明显改善上述结果;苦菊提取物能改善CCl4致小鼠肝急性损伤的肝细胞坏死和炎症病变,并可降低血清中ALT和AST水平,呈剂量依赖趋势,提示苦菊提取物具有肝保护的作用;苦菊提取物对鼠伤寒沙门氏菌株无致基因突变作用。结论苦菊提取物具有良好的肝保护作用,并初步判断其毒性不明显,且无致突变作用。

Objective To investigate the protective effect of Cichorium endivia L. extract （CEE） on injured liver in vitro and in vivo, and the mutagenesis of CEE. Methods The viability and levels of the reactive oxygen species （ROS） in H epG2 cells exposed to 2,2＇-Azobis （2-methylpropionamidine） dihy- drochloride （AAPH） was measured by MTT assay and DCFH-DA fluorescence dye assay, respectively. The carbon tetrachloride （CC14） induced acute liver injury model in mice was used to evaluate the hepatic histopathological change and the levels of serum ALT and AST. The mutagenesis of CEE was assessed by Ames test. Results Treatment of HepG2 cells with AAPH reduced the cell viability and increased intracellular ROS levels, those effects could be reversed by addition of CEE. The hepatocyte necrosis and inflammatory change in acute liver injury mice induced by CC14 were improved by administration of CEE, the ALT and AST levels in the mice serum were reduced in a dose-dependent manner, indicating that CEE had the capacity of hepatoprotection. CEE did not show mutagenic effect as detected in Ames test. Conclusion CEE effectively protects the liver from the injury with little toxicity and no mutagenicity.