α-突触核蛋白和硝基化α-突触核蛋白在不同年龄段食蟹猴结肠中的表达

Differential expressions of α-synuclein and nitrated α-synuclein in colonic in different ages of cynomolgus monkeys

目的研究α-突触核蛋白(α-synuclein,α-syn)及硝基化α-突触核蛋白(nitratedα-synuclein,n-syn)在不同年龄段食蟹猴结肠中的表达。方法免疫组织化学方法检测α-syn和n-syn在不同年龄段食蟹猴结肠中的表达。免疫荧光双标观察α-syn和n-syn的共定位。结果α-Syn和n-syn在食蟹猴结肠中存在共定位。α-Syn和n-syn在不同年龄段食蟹猴结肠中均有表达,但α-syn和n-syn在老年食蟹猴表达量最高,在青年食蟹猴表达量最低。结论α-Syn和n-syn在食蟹猴结肠中存在共定位,且在老年猴中表达最高。

Objective To explore the expressions of α-synuclein（α-syn） and nitratedα-synuclein（ n-syn） in colonic in different ages of cynomolgus monkeys. Methods Immunohis-tochemistry was applied to measure the expressions of α-syn and n-syn in colonic in different ages of cynomolgus monkeys. Double immunofluorescent labeling was used to examine the co-localization of α-syn and n-syn. Results α-Syn and n-syn were colocalized in colonic of cynomolgus monkeys. α-syn and n-syn were expressed in colonic in different ages of cynomolgus monkeys. However,α-syn and n-syn expressions were the highest in colonic of old monkeys and the lowest in colonic of young monkeys. Conclusion α-Syn and n-syn are colocolized in colonic of cynomolgus monkeys and are the highest in colonic of old cynomolgus monkeys.