microRNA146a的单核苷酸多态性与肝细胞性肝癌易感性和切除术后早期复发的关系

The association of the SNP in miRNA146a with genetic prediposion and the earlier recurrence after resection for hepatocellular carcinoma

目的探讨miRNA146a单核苷酸多态性（SNP）位点与肝细胞性癌（HCC）肿瘤遗传易感性以及术后早期复发的关系。方法采用病例对照研究设计，提取173例HCC患者石蜡切片组织DNA，PCR扩增并查找包括miRNA146a外显子在内共386个碱基序列内的SNP位点，分析其与易感性及术后早期复发的关系。结果上述序列中只找到hsa－mir－146a rs2910164G〉C位点，基因库中标注的其他9个SNP位点均未发现。miRNA146aSNPC／C型61例（35．3％）、G／G型26例（15．0％）、C／G型86例（49．7％）。C／C型和C／G型相对G／G型为肝癌的危险因素（OR=3．086，95％CI,1．289—7．390）；miRNA146aSNP（C／G基因型）为肝癌早期复发的危险因素（OR=8．179，95％CI，2．248—29．759）。结论rs2910164位点可能与江西汉族人群肝癌遗传易感性以及术后早期复发相关。

Objective To investigate the association of the SNP in miRNA146A with genetic prediposion and earlier recurrence after resection for hepatocellular carcinoma （HCC）. Methods In the casecontrol study including 173 HCC cases, DNA were exacted from cancer tissue embedded with paraffin and were amplificated by PCR, SNP was explored in gene sequence of miRNA146a （385 base pair including extron）. The outcome were analyzed with genetic prediposion and clinical features. Result Only hsa-mir-146a rs2910164 was found. The genetype frequence of C/C ,G/G and C/G at rs2910164 gene locus were separately 61 （35.3%）,21 （15%） and 86（49.7% ） in cases. Compared to G/G genetype, C/C and C/G genetype were danger factor to onset risk of HCC （ OR = 3. 086,95% CI： 1. 289-7. 390） ; C/G was danger factor to earlier recurrence after resection（OR = 8. 179,95% CI：2. 248-29. 759）. Conclusion rs2910164 may be associated with genetic prediposion and earlier recurrence after resection of HCC in Jiangxi hans