Ras-相互作用蛋白1在肝细胞癌中的表达及其侵袭抑制作用

Expression of RASIP1 in hepatocellular carcinoma and its role in suppressing invasion

目的检测肝细胞癌（HCC）组织标本及细胞系中Ras-相互作用蛋白1（RASIP1）的mRNA和蛋白水平的表达情况，分析其在不同组织及细胞系中表达差异的意义。方法利用实时PCR及蛋白印迹技术检测RASIP1在29对肝癌组织及相应邻近非癌组织标本、四种细胞系（LO2、HepG2、MHCC97-H和HCCLM3）中的表达差异。结果RASIP1 mRNA及蛋白质水平在93．2％（27例）的HCC中呈低表达；正常肝细胞株LO2中RASIP1的mRNA及蛋白水平表达含量明显高于肝癌细胞株（P〈0．05）；高侵袭转移能力的细胞系MHCC97-H以及HCCLM3只有极低的RASIP1表达，且明显低于低侵袭转移能力的细胞系HepG2（P〈0．05）。结论RASIP1在肝癌中的表达水平与其侵袭能力负相关，推测RASIP1可能具有抑制HCC侵袭转移的作用。

Objective To explore the expression levels of RAS-interacting protein 1 （ RASIP1 ） mRNA and protein in hepatocellular carcinoma （HCC） tissues and its cell lines, and to analyze the relationship between RASIP1 and tumorigenesis and progression of hepatocellular carcinoma. Methods The expression levels of RASIP1 mRNA and protein in 29 hepatocellular carcinoma tissues and the corresponding adjacent non-cancer hver tissues （ANLTs） , as well as those in the HCC cell lines such as LO2, HEPG2, MHCC97-H and HCCLM3 were detected using real-time PCR and western blot. Results The RASIP1 expression levels decreased significantly in HCC tissues when compared with the corresponding ANLTs; The expression levels of RASIP1 mRNA and protein in LO2 were significantly higher than those in other HCC cell lines （P 〈 0. 05） ; The expression levels of RASIP1 mRNA and protein in MHCC97-H and HCCLM3 were significantly lower than those in HepG2 （P 〈 0. 05 ）. Conclusions HCC tissues had lower expression than those in ANLTs. On analyzing the RASIP1 levels of HCC tissues and its cell lines, we speculated that RASIP1 might suppress recurrence and metastasis of HCC.