摘要
以披针叶黄华(Thermopsis lanceolata)试管苗为材料,通过组培方法研究其在0、0.2%、0.4%、0.6%、0.8%和1.0%NaCl和Na2SO4胁迫30d后的生长、有机渗透调节物质和无机渗透调节物质(Na+、K+和Ca2+)含量的变化,以探讨其耐盐性机制。结果显示:(1)随NaCl和Na2SO4胁迫浓度的增加,披针叶黄华试管苗叶片脯氨酸和可溶性糖含量均显著持续增加,且NaCl胁迫下脯氨酸上升的幅度均大于相同浓度Na2SO4胁迫下的增幅,而可溶性糖上升的幅度却小于相同浓度Na2SO4胁迫下的幅度;可溶性蛋白含量随NaCl浓度的增大呈先升高后降低的趋势,但随Na2SO4浓度的增加呈持续上升的趋势。(2)随NaCl和Na2SO4浓度的增加,披针叶黄华试管苗Na+含量呈增加趋势且各处理均显著高于对照,Ca2+含量和叶片K+含量却呈逐渐减少趋势且各处理均显著低于对照,而根系K+含量呈先降后升的趋势;Na2SO4胁迫下披针叶黄华试管苗叶片Na+含量上升幅度以及K+和Ca2+含量下降幅度均明显低于相同浓度NaCl胁迫组;而Na+/K+和Na+/Ca2+比值随NaCl和Na2SO4浓度增加而升高;NaCl胁迫下,叶片Na+/K+和Na+/Ca2+高于相同浓度Na2SO4胁迫下的比值,而根系Na+/K+和Na+/Ca2+却低于相同浓度Na2SO4胁迫下的比值。研究表明,盐胁迫下,披针叶黄华试管苗通过抑制叶片中Na+积累并增加可溶性糖和可溶性蛋白含量,在根系中维持较高K+和Ca2+含量以及较低水平Na+/K+和Na+/Ca2+比,以降低披针叶黄华细胞渗透势来适应盐渍环境;披针叶黄华对NaCl胁迫的调节能力弱于Na2SO4。
To study the salt-tolerance mechanism of Thermopsis lanceolata,we determined the growth parameters,organic osmotic adjustment substance contents and inorganic ions such as Na+,K+ and Ca2+ cation contents of T.lanceolata test-tube plantlets which have been growing for 30 d under 0,0.2%,0.4%,0.6%,0.8%,1.0% NaCl and Na2SO4 Stress.The results showed:(1)With NaCl and Na2SO4 concentration increasing,the contents of free proline and soluble sugar in leaves of T.lanceolata test-tube plantlets increased significantly,but the increasing extent of free proline under NaCl stress was greater than that under the same Na2SO4 concentration stress,while the increasing extent of soluble sugar content under NaCl stress was smaller than that under the same Na2SO4 concentration stress;the soluble protein content increased first then decreased with the increasing of NaCl concentration while went up continuously with the increasing of Na2SO4 concentration.(2)Na+ content of T.lanceolata test-tube plantlets went up continuously with the increased of the NaCl and Na2SO4 concentrations and the Na+ contents of all treatments were significantly higher than those in the control.Ca2+ and K+ content in leaves presented a decreasing trend,which were significantly lower than that of the control,respectively.In roots,K+ content first increased and then declined.Compared with NaCl,Na2SO4 stress caused a lower increase of Na+ content and lower reduction of K+ and Ca2+ contents in leaves;Na+/K+and Na+/Ca2+ ratios increased with the increase of salt content;In leaves,Na+/K+and Na+/Ca2+ ratios under NaCl stress were higher than that under Na2SO4 stress,while Na+/K+and Na+/Ca2+ ratios in roots were lower than that under Na2SO4 stress.T.lanceolata test-tube plantlets under salt stress may adapt to salt environment depressing Na+ contents,increasing soluble sugar and soluble protein contents in leaves,higher K+ and Ca2+ contents and decreased Na+/K+and Na+/Ca2+ ratios in roots.In words,the adaptation of T.lanceolata to NaCl stress was smaller than that to Na2SO4 stress.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第5期963-969,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
河西学院科研创新与应用校长基金项目(hxxyxzjj-201003)
关键词
盐胁迫
披针叶黄华
渗透调节
离子含量与比例
salt stress
Thermopsis lanceolata
osmotic adjustment
cation content and ratio