半夏不同炮制品HPLC特征指纹图谱的研究

Research on the HPLC Characteristic Fingerprints of Rhizoma Pinelliae and Processed Products

目的对半夏及其炮制品清半夏、姜半夏、法半夏、京半夏不同部位HPLC特征指纹图谱进行研究,为有效控制半夏及其不同炮制品质量提供科学依据。方法采用HPLC梯度洗脱建立半夏及其不同炮制品的水、体积分数75%、95%乙醇部位的特征指纹图谱,运用"中药色谱指纹图谱相似度评价系统2004A版"进行分析。结果分别建立了半夏及其炮制品不同部位的HPLC特征指纹图谱及共有模式图,半夏、清半夏的共有峰为13个,姜半夏的共有峰为15个,法半夏、京半夏的共有峰为17个,分别对肌苷、鸟苷、腺苷、琥珀酸、盐酸麻黄碱、甘草苷、甘草酸铵、6-姜辣素8个特征峰进行了指认。半夏及其4种炮制品均具有鸟苷、腺苷、琥珀酸、盐酸麻黄碱4个特征峰;6-姜辣素为姜半夏独有特征峰;甘草苷、甘草酸铵为法半夏、京半夏独有特征峰。此外,与半夏相比,清半夏、姜半夏新增1个未知成分(8号峰,18.3 min);法半夏、京半夏缺失肌苷,新增1个未知成分(11号峰,45.4 min)。结论本实验建立了半夏及其炮制品清半夏、姜半夏、法半夏、京半夏不同部位HPLC特征指纹图谱,该方法稳定、简便、可靠,可有效鉴别半夏及其4种炮制品,同时为其质量控制提供一定的科学依据。

OBJECTIVE To establish and compare the HPLC fingerprints of different parts of Rhizoma Pinelliae （ RPI）, Rhizoma Pinelliae Praeparatum Cum Alumine （RPA）, Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine （RPZ）, Rhizoma Pinelliae Praeparatum （RPP） and Jing Pinelliae （JPI） and provide reliable method and scientific basis for their quality control. METHODS The HPLC fingerprints of water, 75% ethanol and 95% ethanol extracts of Rhizoma Pinelliae and processed products were established and analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （ Version 2004A）. RESULTS The chromatograms of water, 75% ethanol and 95% ethanol extracts were generated as the representative standard fingerprints. Thirteen common peaks were obtained in the fingerprints of Rhizoma Pinelliae and Rhizoma Pinelliae Praeparatum Cum Alumine; 15 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; 17 common peaks were obtained in the fingerprints of Rhizoma Pinelliae Praeparatum and Jing Pinelliae. Among the common peaks, 8 characterized peaks were identified as inosine, guanosine, adenosine, succinic acid, ephedrine hydrochloride, liquiritin, glycyrrhizic acid, and 6-Gingerol. Guanosine, adenosine, succinic acid, and ephedrine hydrochloride existed in Rhizoma Pinelliae and processed products. 6-gingerol was only detected in Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine. Liquiritin and glycyrrhizic acid were detected in Rhizoma Pinelliae Praeparatum and Jing Pinelliae. A new peak （ peak 8） appeared in the chromatograms of Rhizoma Pinelliae Praeparatum Cum Alumine and Rhizoma Pinelliae Praeparatum Cum Zingibere Et Alumine; Rhizoma Pinelliae Praeparatum and Jing Pinelliae did not show inosine and had a new peak （peak 11 ） compared to Rhizoma Pinelliae. CONCLUSION This study established the HPLC characteristic fingerprints of different parts of Rhizoma Pinelliae and processed products. The method is stable, time-saving, reliable and can identify Rhizoma Pinelliae and its four different processed products, which provides a scientific basis for the quality control of Rhizoma Pinelliae.