血液灌流对灭多威中毒兔中枢神经系统保护作用研究

Protective effect of hemoperfusion on central nervous system of rabbits with methomyl poisoning

目的观察血液灌流(HP)对灭多威中毒兔脑组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量影响。方法 24只兔随机分成正常对照组(N组)、中毒组(P组)、血液灌流组(HP组),每组8只,建立HP治疗兔灭多威中毒模型,分别测定各组兔脑组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量变化,并观察脑组织病理学改变。结果 P组实验兔脑组织SOD活性较HP组、N组明显下降,差异有统计学意义(P<0.05),MDA含量较HP组、N组明显升高,差异有统计学意义(P<0.05);光学显微镜观察下,P组脑组织可见充血、水肿等病理学改变,HP组与N组脑组织充血水肿等病理改变不明显。结论早期血液灌流可减轻灭多威中毒所致脑组织氧化应激损伤,对中枢神经系统有一定保护作用。

Objective To observe the effcet of hemoperfusion on SOD and MDA of brain tissue under acute toxication of methomyl in rabbits. Methods Twenty-four rabbits were randomly divided into three groups： control group（ N group）,acute methomyl intoxication group（ P group） and hemoperfusion group（ HP group）. The activity of SOD and the content of MDA in brain tissue were measured by spectrophotography,the histopathological changes of brain were examined. Results The activity of SOD was significantly increased while MDA content was decreased in HP group as compared to in P group（ P 0. 05）,There was no significant difference in each index between HP group and N group（ P 0. 05）. Conclusion Hemoperfusion can attenuate the brain injury caused by methomyl poisoning through suppresses the production of MDA,and improve SOD activity.