没食子酸乙酯对脓毒症致大鼠急性肺损伤的影响

Effects of ethyl gallate on sepsis-induced acute lung injury in rats

目的：评价没食子酸乙酯（EG ）对脓毒症致大鼠急性肺损伤（ALI ）的影响。方法选取健康雄性Wistar大鼠48只，10～12周龄，体重150～250 g ，采用盲肠结扎穿孔法建立脓毒症模型。采用随机数字表法，将其分为3组（ n＝16）：对照组（C组）、ALI组造模后6 h时经股静脉输注生理盐水1 ml/h ，持续时间4 h；EG组造模后6 h时经股静脉输注没食子酸乙酯1 ml/h（50 mg·kg-1·h-1），持续时间4 h。于造模后10 h （T1）和24 h （T2）时处死大鼠，取肺组织，检测肺组织湿/干重（W/D ）比值、SOD活性、MDA和还原型谷胱甘肽（GSH ）的含量，采用免疫组化法检测肺组织三硝基酪氨酸（3-NT ）表达水平，光镜下观察肺组织病理学结果。结果与C组比较，ALI组T1，2时肺组织W/D比值、MDA含量、3-NT表达水平升高，GSH含量降低，T2时肺组织SOD活性降低，EG组T1时肺组织MDA含量、3-NT表达水平升高，T2时肺组织W/D比值、MDA含量、3-NT表达水平升高，GSH含量降低（ P＜0.05）。与ALI组比较，EG组T1时肺组织MDA含量降低，T2时肺组织W/D比值、MDA含量、3-NT表达水平降低，GSH含量和SOD活性升高（ P＜0.05）。ALI组肺组织病理损伤明显，EG组肺组织病理损伤程度明显减轻。结论 EG可减轻脓毒症致大鼠ALI ，其机制与其抗氧化作用有关。

Objective To evaluate the effects of ethyl gallate （EG ） on sepsis-induced acute lung injury （ALI） in rats .Methods Forty-eight healthy male Wistar rats ,aged 10-12 weeks ,weighing 150-250 g ,were randomly divided into 3 groups （n=16 each） using a random number table ：control group （group C） ,group ALI , and group EG .The animals were anesthetized with intraperitoneal pentobarbital sodium 50 mg/kg .Sepsis was induced by cecal ligation and puncture （CLP） .EG 1 ml/h （50 mg·kg-1 ·h-1 ） was infused intravenously via the femoral vein for 4 h starting from 6 h after CLP in group EG .The equal volume of normal saline was given instead in group ALI . The rats were sacrificed at 10 and 24 h after CLP （T1 ,2 ） , and the lungs were removed for determination of wet/dry lung weight ratio （W/D ratio ） , superoxide dismutase （SOD ） activity , contents of malondialdehyde （MDA ） and glutathione （GSH ） , and 3-NT expression （by immuno-histochemistry ） in lung tissues .The pathological changes of the lung were examined with light microscope .Results Compared with group C ,the W/D ratio ,MDA content and 3-NT expression were significantly increased and GSH content was decreased at T1 ,2 ,the SOD activity was significantly decreased at T2 in group ALI ,and the MDA content and 3-NT expression were increased at T1 ,and the W/D ratio ,MDA content and 3-NT expression were increased and GSH content was decreased at T2 in group EG （ P〈0.05） .Compared with group ALI ,the MDA content was significantly decreased at T1 ,and the W/D ratio ,MDA content and 3-NT expression were decreased ,and GSH content and SOD activity＆nbsp;were increased at T2 in group EG （ P〈0.05） .The pathological changes of the lung were obvious in group ALI , and significantly attenuated in group EG .Conclusion EG can attenuate sepsis-induced ALI by antioxidative effects in rats .