不同剂量异丙酚对大鼠创伤性脑损伤的影响

Effects of different doses of propofol on traumatic brain injury in rats

目的：评价不同剂量异丙酚对大鼠创伤性脑损伤的影响。方法清洁级健康雄性SD大鼠48只，周龄7～8周，体重270～320 g ，采用随机数字表法分为6组（ n＝8）。采用改进Feeney法自由落体脑损伤装置制备大鼠创伤性脑损伤模型。S组仅开窗后用骨蜡封闭，左侧股静脉输注0.9％生理盐水3.49 ml·kg-1·h-1；模型制备成功后，创伤性脑损伤组（I组）、脂肪乳组（F组）、低剂量异丙酚组（L组）、中剂量异丙酚组（M组）和高剂量异丙酚组（H组）分别经左侧股静脉输注0.9％生理盐水、20％脂肪乳3.49 ml·kg-1·h-1、异丙酚17.46、34.92和69.84 mg·kg-1·h-1。于输注15 min（T1）和60 min （T2）时取右侧颈总动脉血，测定血清S100β蛋白浓度，T2时取血后处死大鼠取脑，光镜下观察大脑皮质病理学结果，透射电镜下观察大脑皮质神经细胞超微结构。结果与S组比较，其余组T1，2时血清S100β蛋白浓度升高（ P＜0.05）；与I组和F组比较，L组、M组和H组T1，2时血清S100β蛋白浓度降低（ P＜0.05）；与L组比较，M组和H组T2时血清S100β蛋白浓度降低，H组T1时血清S100β蛋白浓度升高（ P＜0.05）；与M组比较，H组T1，2时血清S100β蛋白浓度升高（ P＜0.05）。光镜结果：异丙酚各剂量组细胞核固缩、神经细胞变性坏死、细胞水肿程度等较I组减轻，M组可见正常神经细胞。透射电镜结果：异丙酚各剂量组细胞核膜内陷、线粒体空泡样变、粗面内质网扩张、染色质聚集或边集及突触前、后膜和间隙等较I组损伤程度减轻。结论不同剂量异丙酚可减轻大鼠创伤性脑损伤。

Objective To evaluate the effects of different doses of propofol on traumatic brain injury in rats .Methods Forty-eight healthy male Sprague-Dawley rats , aged 7-8 weeks , weighing 270-320 g , were randomly divided into 6 groups （ n=8 each ） using a random number table ：sham operation group （group S ） , traumatic brain injury group （group I） ,fat emulsion group （group F） and low-dose propofol group （group L） , medium-dose propofol group （group M ） ,and high-dose propofol group （group H ） .Traumatic brain injury model was established according to the method described by Feeney .In group S ,0.9% normal saline was infused into the left femoral vein at 3.49 ml·kg-1 ·h-1 .In I ,F ,L ,M and H groups ,0.9% normal saline ,20% fat emulsion 3.49 ml·kg-1 ·h-1 ,and propofol 17.46 ,34.92 and 69.84 mg·kg-1 ·h-1 were infused into the left femoral vein ,respectively .Blood samples were collected from the right common carotid artery at 15 and 60 min of infusion （T1-2 ） for determination of serum S100β protein concentrations . The rats were then sacrificed after collecting blood samples at T2 and brains were removed for microscopic examination of the pathological changes of the cerebral cortex （light microscope ） and ultrastructure of neurons in the cerebral cortex （transmission electron microscope） .Results The serum S100β protein concentrations were significantly higher at T1 ,2 in the other five groups than in group S （ P〈0.05 ） .Compared with I and F groups ,the serum S100βprotein concentrations were＆nbsp;significantly decreased at T1 ,2 in L ,M and H groups （ P〈0.05） .Compared with group L ,the serum S100βprotein concentrations were significantly decreased at T2 in M and H groups , and the serum S100β protein concentrations were increased at T1 in H group （ P〈 0.05 ） . The serum S100β protein concentrations were significantly higher at T1 ,2 in H group than in group M （ P〈0.05 ） .Light microscopic examination showed that nucleus condensation , cell necrosis , and cell edema were significantly attenuated in L ,M , and H groups as compared with group I;normal neurons could be found in group M .Transmission electron microscopic examination showed that the severity of neuronal damage was significantly attenuated in L ,M ,and H groups as compared with group I .Conclusion Different doses of propofol can reduce traumatic brain injury in rats .