Jagged1、VEGF 与人脑胶质瘤的关系

Relationship between Jagged1,VEGF and human brain gliomas

目的：探讨 Jagged1、VEGF 在人脑胶质瘤中的表达。方法收集42例人脑胶质瘤（Ⅰ级9例、Ⅱ级11例、Ⅲ级14例、Ⅳ级8例）及6例正常脑组织标本，采用半定量逆转录聚合酶链式反应技术（RT-PCR）检测肿瘤组和正常组脑组织中 Jagged1的 mRNA 表达水平，用酶联免疫吸附法（ELISA）测定两组患者血清中 VEGF 水平。结果①与正常组相比，肿瘤组 Jagged1 mRNA 表达水平显著升高，差异有统计学意义（P 〈0.05）；Ⅲ级与Ⅳ级比较差异无统计学意义（P ＝0.061），其余两两比较结果差异均具有统计学意义（P 〈0.05）。②与正常组相比，肿瘤组血清 VEGF 表达水平显著升高，差异有统计学意义（P 〈0.05）；Ⅰ级与Ⅱ级比较结果无明显差异（P ＝0.239），Ⅲ级与Ⅳ级比较差异无统计学意义（P ＝0.089），其余结果两两比较差异均具有统计学意义（P 〈0.05）。③胶质瘤患者Jagged1 mRNA 表达水平与血清 VEGF 浓度呈明显正相关（r ＝0.986，P ＝0.014）。结论 Jagged1可能参与了人脑胶质瘤血管的生成，有望作为抗肿瘤治疗的新靶点。

Objective To investigate the expression levels of Jagged1 and VEGF in human brain gliomas. Methods Forty-two cases of human brain gliomas（9 cases in gradeⅠ,11 cases in grade Ⅱ,14 cases in gradeⅢ,8 cases in gradeⅣ）and 6 cases of normal brain tissues were collected after operation. The expression of Jagged1 mRNA was determined by RT-PCR assay. The VEGF level was measured by enzyme linked immunosorbent assay（ELISA）. Results ①The expression of Jagged1 mRNA significant-ly increased in human brain gliomas than that in normal brain tissues（P 〈 0. 05）,there was no signifi-cant difference between grade Ⅲ and grade Ⅳ in the expression of Jagged1 mRNA（ P = 0. 061）, and the differences between other grades were significant（ P 〈 0. 05）. ②The VEGF level significant-ly increased in human brain gliomas than that in normal brain group（ P 〈 0. 05）;There was no signif-icant difference between grade Ⅰ and grade Ⅱ（ P = 0. 239 ）,so did grade Ⅲ and grade Ⅳ（ P =0. 089）,the differences between other grades were significant（ P 〈 0. 05）. ③The expression of Jag-ged1 mRNA was positively correlated with the level of VEGF（ r = 0. 986,P = 0. 014 ）. Conclu-sions Jagged1 may take part in human brain gliomas angiogenesis and it may be a novel target for antiangiogenic therapy.