秦岭玄参与玄参的生药学和抗炎镇痛活性比较研究

Comparative study on the pharmacognosy and anti-inflammatory activities of Scrophularia buergeriana Miq.var.tsinglingensis Tsoong and S.ningpoensis Hemsl.

目的：比较秦岭玄参与玄参在生药学和抗炎镇痛方面的差异。方法通过性状观察、组织显微鉴别比较2种植物的生药学差异，应用二甲苯致小鼠耳廓肿胀模型分析二者的抗炎作用，利用热板法和醋酸扭体法观测二者的镇痛差异。结果秦岭玄参和玄参的聚伞花序分别集成顶生穗状花序、圆锥花序；二者原生木质部分别为类圆形或圆形和多角形；前者形成层环不明显、皮层无石细胞；后者形成层环型、石细胞单个散在，或2～5个成群。秦岭玄参和玄参大剂量明显降低二甲苯所致小鼠耳廓肿胀度（P＜0．05）；两者显著提高小鼠痛阈（P＜0．05，P＜0．01），减少小鼠扭体次数（P＜0．05，P＜0．01）。结论秦岭玄参与玄参生药学在花序、毛茸、原生木质部、形成层及石细胞等方面存在差异；药理研究表明，秦岭玄参均有抗炎和镇痛作用，且可以替代常用的玄参入药。

Objective To compare the pharmacognosy and anti-inflammatory activities of Scrophularia buergeriana Miq .var .tsin-glingensis Tsoong and S .ningpoensis Hemsl ..Methods The traits and microscopic characteristics were used to study the pharma-cognosical differences of the two plants .The ear swelling model induced by xylene in mice was employed for the analysis of anti-inflammatory effects .Hot plate test and acetic acid writhing approaches were used to observe the analgesic effects .Results Cymes of Scrophularia buergeriana Miq .var .tsinglingensis Tsoong and S .ningpoensis Hemsl .exhibited spike and panicle on the top , respectively .Primary xylems of both plants were round or polygon .The former had no apparent cambium ,or no stone cells in cor-tex .The later had apparent cambium and contained single stone cell or 2-5 in clustering .High dose of Scrophularia buergeriana Miq .var .tsinglingensis Tsoong and S .ningpoensis Hemsl .obviously reduced the ear swelling intensity induced by xylene in mice＆amp;nbsp;（P＆lt;0 .05） .Both could significantly enhance the mice pain threshold（P＆lt;0 .05 ,P＆lt;0 .01） and decrease the mice writhing times（P＆lt;0 .05 ,P＆lt;0 .01） .Conclusion Scrophularia buergeriana Miq .var .tsinglingensis Tsoong was different in pharmacognosy with S .ningpoensis Hemsl .,at aspects of inflorescence ,hairy ,primary xylem ,cambium and stone cells ,etc .The pharmacological re-search suggests that Scrophularia buergeriana Miq .var .tsinglingensis Tsoong have the anti-inflammatory and analgesic effects and is an alternative for medication replacing the commonly used Scrophularia ningpoensis Hemsl ..