非小细胞肺癌中p16基因甲基化的临床分析

Clinical Analysis of Methylation of p16 Gene in Non-small Cell Lung Cancer

目的：研究非小细胞肺癌中p16基因甲基化的表达，分析p16基因甲基化与临床、病理资料之间的相关性。方法对61例经手术切除且病理确诊的非小细胞肺癌在术中提取新鲜癌组织和癌旁组织样本冷冻保存，提取DNA后经重亚硫酸盐修饰，用甲基化特异性PCR（MSP）方法检测癌和癌旁组织中p16基因甲基化状态。结合临床及病理资料对实验结果进行统计学分析。结果非小细胞肺癌和癌旁组织中出现p16基因甲基化的阳性率分别为11.48%（7/61）和1.64%（1/61）（P＞0.05）。不同的病理分型、分化程度、TNM分期、淋巴结转移程度及吸烟指数间p16基因甲基化状态差异无统计学意义（P＞0.05）。结论非小细胞肺癌组织中存在p16基因甲基化。

Objective To investigate methylation of p16 gene in non-small cell lung cancer （NSCLC）, and analyze the correlation between it and clinical and pathological data. Methods Specimens were taken from 61 NSCLC patients pathological conifrmed after surgical resection. Fresh tumor samples matched with corresponding adjacent normal tissue were taken during operation and frozen preserved. After extraction and Bisulifte treatment of DNA from the frozen tissues, we examined the methylation of p16 gene in NSCLC tissues and corresponding paracancerous tissues by using methylation-speciifc PCR （MSP） method. Then the result of experiment was ana-lyzed statistically combined with clinical and pathological data. Results The positive rate of the methylation of p16 gene was respectively 11.48%（7/61） in lung cancer tissues and 1.64%（1/61） in paracancerous tissues （P〉0.05）. No signiifcant difference was found in the methylation of p16 gene according to different pathologic classi-ifcation, differentiation of cancer, TNM stage, the extent of metastasis of lymph node and smoking index （P〉0.05）. Conclusion The methylation of p16 gene existed in NSCLC tissues.