七氟烷对LPS诱导小鼠急性肺损伤的保护作用及机理

The Protective Effect of Sevolfurane to LPS Induced Acute Lung Injury and the Underlying Mechanism

目的：探讨七氟烷对脂多糖（LPS）诱导肺部炎性损伤的保护作用及机理。方法将C57L/B6小鼠置于分别置于密闭的玻璃容器内，吸入七氟烷后腹腔注射LPS（Sevo＋LPS组），6 h后处死小鼠大体观察肺部改变、测量肺重和肺湿重/干重比（W/D），HE染色观察小鼠肺部病理变化。心脏采血后检测血清中IFN-γ和IL-10水平变化。设立吸入氧后腹腔注射LPS组（O2＋LPS组）和吸入氧后腹腔注射PBS组（O2＋PBS组）作为对照。结果七氟烷吸入可减轻LPS诱导小鼠肺部水肿， O2＋PBS组W/D值为3.96±0.23；O2＋LPS组W/D值为5.73±0.43，与O2＋PBS组相比明显增高（P＜0.01）；Sevo＋LPS组W/D值为4.62±0.41，比O2＋LPS组明显降低（P＜0.01）。HE染色肺组织切片发现七氟烷吸入可减轻LPS诱导小鼠肺组织水肿和渗出。七氟烷吸入可降低小鼠血清IFN-γ水平和提高血清IL-10的水平。结论七氟烷可能通过改变IFN-γ和IL-10等细胞因子平衡减轻LPS诱导的肺部炎性损伤。

Objective To explore the protective effect of sevolfurane to LPS induced acute lung injury and the underlying mechanism. Methods C57L/B6 mice were put into a sealed glass chamber administrated LPS with intraperitoneal injection after inhaled sevolfurane （Sevo＋LPS group）. 6h later, mice were sacriifced to observe the pathological changes, measure the weight of lung and calculate the wet weight/dry weight ratio of lung. And then, the serum levels of IFN-γand IL-10 were assayed with ELISA after hemospasia from heart. Administrated LPS with intraperitoneal injection after inhaled oxygen （O2＋LPS group） and administrated PBS with intraperitoneal injection after inhaled oxygen （O2＋PBS group） were set up as control groups. Results Sevolfurane could relief the LPS induced lung edema. The W/D ratio of O2＋PBS group was 3.96±0.23, and the W/D ratio of O2＋LPS group was 5.73±0.43. And the W/D ratio of Sevo＋LPS group was 4.62±0.41, less than that of O2＋LPS group （P〈0.01）. Lung tissue stained with HE indicated that sevolfurane could decrease the edema and exudation of lung. ELISA assay showed that sevolfurane could inhibit the serum level of IFN-γand enhanced the serum level of IL-10. Con-clusion Sevolfurane might relief the lung inlfammatory injury induced with LPS by altering the balance of cyto-kines.