白细胞介素21基因联合放射对肺癌细胞生长的协同抑制作用

Synergism inhibition effect of adenovirus-mediated IL-21 gene combined with ionizing radiation on the growth of pulmonary carcinoma cells

目的：探讨白细胞介素21（IL-21）基因联合放射对肺癌A549细胞生长的协同抑制作用。方法将肺癌A549细胞分为4组：空白对照组、Ad-IL-21组（用Ad-IL-21腺病毒转染细胞）、照射组（行6 Gy 137Csγ射线照射）以及Ad-IL-21联合照射组（用Ad-IL-21腺病毒转染细胞后再行6 Gy 137Csγ射线照射），观察IL-21基因对A549细胞生长的抑制效应。结果 Ad-IL-21联合照射组的A549细胞的抑制效应最强（抑制率约为40%），显著高于Ad-IL-21组（约22%）和照射组（约17%）（F=45．6、57．5，P＜0．05）。Ad-IL-21联合照射组A549细胞发生明显的G2期阻滞（35．4%），与Ad-IL-21组（25．2%）和照射组（17．5%）比较，差异有统计学意义（F=16．6、32．8， P＜0．05）。Ad-IL-21联合照射组凋亡细胞所占比例最高，凋亡率达到33．1%，与Ad-IL-21组（27．8%）和照射组（14．8%）相比差异有统计学意义（F=15．9、41．7，P＜0．05）。结论 IL-21基因联合放射对肺癌细胞生长的抑制效应具有协同作用，可显著地抑制肿瘤细胞的生长。

Objective To explore the synergism inhibition effect of adenovirus-mediated inter-leukin-21 （IL-21） gene combined with ionizing radiation on the growth of pulmonary carcinoma cells A549. Methods A549 cells were divided into four groups： blank control group , Ad-IL-21 group （cells were transfected with Ad-IL-21）, radiation group （cells were exposed to 6 Gy137Cs γ-ray） and Ad-IL-21 combined with radiation group （ cells were transfected with Ad-IL-21 before exposed to 6 Gy 137Cs γ-ray）. The cell growth, cell cycle and cell apoptosis of A549 cells were observed. Results The inhibition rate of Ad-IL-21 combined with radiation group was the highest, reached 40%, which was significantly higher than that of Ad-IL-21 group （about 22%）and radiation group （about17%） （F=45.6 and 57.5, both P〈0.05）. Cells of Ad-IL-21 combined with radiation group were arrested at G2 phase（35.4%）, significantly higher than that of Ad-IL-21 group（25.2%）and radiation group（17.5%） （F=16.6 and 32.8, both P〈0.05）. The cell apoptosis rate of combination group was the highest, reached 33.1%and there were significant differences compared with Ad-IL-21 group （27.8%）and ra-diation group （14.8%）（F=15.9 and 41.7, both P〈0.05）. Conclusion Ad-IL-21 transfection com-bined with radiation shows synergism effect on the inhibition of pulmonary carcinoma cells growth .