津力达对糖尿病大鼠血糖调节激素及胰岛细胞功能干预作用研究

Effect of Jin Li Da's intervention of blood glucose regulation hormone and pancreatic islet cell function in diabetic rats

目的:观察2型糖尿病大鼠血清胰岛素、胰高血糖素、生长激素及胰岛内分泌细胞表达变化,探讨中药津力达颗粒(JLD)对血糖调节激素及胰岛细胞功能的干预作用。方法:采用高脂饲料及腹腔注射链脲佐菌素(45 mg/kg)诱导2型糖尿病大鼠模型,继续高脂饲养并给予津力达、罗格列酮干预,8周后检测血清胰岛素、胰高血糖素及生长激素水平,并对胰岛进行免疫组织化学染色及胰岛内分泌激素表达半定量分析。结果:津力达干预能够降低空腹血糖、血清胰高血糖素及生长激素水平(P<0.05,P<0.01),升高血清胰岛素水平(P<0.05),增加胰岛内胰岛素表达,同时降低胰高血糖素表达(P<0.05,P<0.01)。津力达组生长抑素表达略有升高,胰多肽表达有下降趋势,但与模型组比较差异无统计学意义(P>0.05)。结论:津力达颗粒通过调节糖尿病大鼠血糖相关激素水平及胰岛内分泌细胞功能,从而改善糖尿病大鼠糖代谢紊乱。

Objective ： To observe the change of serum insulin, glucagon, growth hormone and expression of pancreatic endocrine cells in type 2 diabetic rat, and study the intervention effect of traditional Chinese medicine Jinlida particles （JLD） on blood glucose regulation-related hormones. Methoe high fat diet and intervene with JLD,ds：Applying the methods which combined high fat diet with STZ （i. p. 45 mg/kg） to establish type 2 diabetes rat model,continu rosiglitazone for eight weeks, detect serum insulin, glucagon and growth hormone levels, and semi-quantitative analysis expression of immunohistochemical staining in islets. Results： The fasting blood glucose, glucagon and growth hormone levels of JLD intervention group were reduced （P 〈 0. 05, P 〈 0. 01 ） , serum insulin level was elevated, and the expression of insulin in islets was significantly increased, while glucagon expression was significantly reduced （ P 〈 0. 05, P 〈 0.01 ）. Somatostatin expression of JLD group was slightly elevated and pancreatic polypeptide expression on a downward trend, but there was no significant difference with the model group. Conclusion： Jinlida particles can improve glucose metabolism in diabetic rats by regulating secretion of blood glucose related-hormone and the function of pancreatic endocrine ceils.