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细胞因子诱导的杀伤细胞对胃癌细胞的杀伤作用的实验研究

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摘要 目的 研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后CIK-DC的抗胃癌细胞活性.方法 正常人外周血单个核细胞诱导培养DC和CIK细胞,再将DC与CIK混合共培养,用台盼蓝活细胞计数计算细胞活率,流式细胞术分析免疫表型.将CIK-DC与胃癌细胞混合,使用MTT法测定对胃癌细胞的杀伤活性.结果 DC、CIK细胞共培养后,与胃癌细胞10:1 ~50:1混合的靶效比范围内,CIK-DC对胃癌细胞的杀伤率显著高于单独的CIK细胞(P<0.05),杀伤率与效靶比有关.结论 CIK-DC的抗胃癌细胞活性高于CIK细胞,是细胞免疫治疗非常有前景的治疗方法.
出处 《中华临床医师杂志(电子版)》 CAS 2013年第11期156-157,共2页 Chinese Journal of Clinicians(Electronic Edition)
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