顺铂抑制IL-8诱导人卵巢癌SKOV-3细胞的迁移作用及相关机制的研究

Mechanisms of DDP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8

目的 观察外源性人白细胞介素-8 （IL-8）对人卵巢癌SKOV-3细胞迁移的影响,及顺铂对它的干预作用,初步探讨顺铂抑制细胞迁移的机制.方法 顺铂处理SKOV-3细胞,MTT法确定顺铂使用的最佳作用浓度；Transwell法观察顺铂对IL-8诱导SKOV-3细胞迁移的干预作用；ELISA法检测顺铂分泌IL-8情况；Western blot法检测NF-κB蛋白表达水平.结果 MTT结果显示：与对照组相比,顺铂浓度为100μg/ml对细胞增殖有显著抑制作用（P＜0.05）.浓度区间在200～400μg/ml的顺铂对细胞的增殖也有抑制作用,该范围内对细胞的生长抑制率无统计学差异（P＞0.05）.浓度为100μg/ml的顺铂,分别作用于SKOV-3细胞24 h、48 h、72 h,各时间组比较无统计学差异（P＞0.05）.IL-8（100 ng/L）处理细胞后,SKOV-3细胞的迁移能力增强,顺铂（100 μg/ml）具有抑制IL-8诱导的SKOV-3细胞迁移的作用,随着浓度的增高,迁移的细胞数由241.67减少到155.99,差异有统计学意义（P＜0.05）.顺铂刺激细胞后,与空白对照组相比,NF-κB蛋白表达水平降低（64.04±4.6）.结论 IL-8具有促进人卵巢癌SKOV-3细胞迁移的作用,顺铂可能是通过NF-κB细胞信号通路对卵巢癌细胞的迁移起抑制作用.

Objective To study the effect of exogenous human interleukin-8 on human ovarian cancer SKOV-3 cell migration and the intervention role of cisplatin on it; to preliminary explore the mechanisms of DDP inhibiting cell migration.Methods SKOV-3 cells were treated with DDP.Optimal concentration and effect time of DDP was determined with MTT assay.Transwell assay were used to study the intervention effect of DDP.Western blot assay was applied to detect NF-κB protein expression levels.Results MTT assay：compared with the control group,the best concentration of DDP which had a significant inhibitory effect on cell proliferation was 100 μg/ml （P 〈0.05）.The concentration of DDP in the range of 200 μg/ml to 400 μg/ml also had the intervention effect,but cell growth inhibition rate between these groups showed no significant difference （P 〉 0.05）.DDP （100 μg/ml）were treated on SKOV-3 cells 24 h,48 h,72 h respectively,the inhibition rate of different times showed no significant difference（P 〉 0.05）.The migratory ability of cells SKOV-3 was increased when IL-8 （100 ng/L）was added in.DD？ （100 μg/ml）inhibited IL-8 induced SKOV-3 cell migration,and as the concentration increased the number of cell migration was reduced from 241.67 to 155.99 （P 〈 0.05）.Cells dealed with DDP,which NF-κB protein expression level were reduced significantly （64.04 ± 4.6）.Conclusion IL-8 can promote the migration of human ovarian cancer cells SKOV-3.DDP can inhibit the migration,and this process probably depends on the activating of the NFκB pathway.