中药血必净对多器官功能障碍综合征大鼠血管性血友病因子和环氧化酶-2及转化生长因子-β1表达的影响及意义

Effect and significance of Chinese herbal medicine on MODS rat vWF,COX-2 and TGF beta 1 expression

目的多器官功能障碍综合征(MODS)是急危重症常见疾病,通过观察活血化瘀中药血必净对MODS大鼠的血管性血友病因子(vWF)、环氧化酶-2(COX-2)、转化生长因子-β1(TGF-β1)表达的影响,探讨其对保护内皮细胞,改善凝血功能,减轻炎症反应的作用。方法将144只SD大鼠(雄性)采用随机化原则分成3组,即假手术对照(对照组)组(48只),MODS(模型组)组(48只),MODS+血必净(实验组)组(48只),每组大鼠再次随机化分为4组,即12h、24h、36h和48h组四个时间点,每组12只大鼠。每组于造模后处死相应时间点动物。取血清用酶联免疫吸附法测定大鼠vWF因子、COX-2因子、TGF-β1因子水平。数据结果采用SPSS17.0统计软件进行统计学分析。结果与对照组相比,模型组及实验组大鼠各时间点的vWF、COX-2和TGF-β1表达水平明显增加,差异有统计学意义(P<0.05);与模型组相比,实验组vWF表达水平于24h起明显下降,TGF-β1和COX-2表达水平在48h明显下降,差异有统计学意义(P<0.05)。结论中药血必净能明显保护MODS大鼠内皮细胞,改善MODS大鼠的凝血功能,减轻MODS大鼠的炎症反应。

Objective Multiple organ dysfunction syndrome（MODS） is an emergency but common disease, this essay is going to discuss the effects of protecting endothelial ceils, improving coagulation function, reducing MODS rat inflammatory reaction of the rat who infected MODS treated by Xuebijing, by observing its influence on MODS rat＇s vWF, COX-2 and TGF-β1. Methods 144 SD rats （male）were divided into three groups at random, namely, sham control group （ comparison group, n = 48）, MODS （ model group, n = 48）, MODS and Xue Bi Jing group （ test group, n = 48 ）, then, each group was divided into four sub-groups at random again, ie, 12 h, 24 h, 36 h and 48 h group of four points,each group had 12 rats. Same number of rats in each sub-group was killed right at the point of 12 h,24 h,36 h and 48 h after membrane came into being. Blood was collected for the determination of enzyme-linked immunosorbent assay vWF factor in rats, COX-2 factor,TGF-β1 in factor. The data were analyzed by SPSS 17.0 statistical software. Results Compared with comparison group,rats in model group and test group had higher vWF, COX-2 and TGF-β1 level in the same measure hour period, the divergence was significant for statistics（ P 〈 0. 05 ） ; compared with model group,vWF level of test group came to descend obviously from 24th hour, the divergence was significant for statistics（P 〈 0. 05）, COX-2 and TGF-β1 level of test group descend obviously in 48th hour, the divergence was significant for statistics （ P 〈 0. 05 ）. Conclusions Chinese medicine Xuehijing could significantly protect endothelial ceils, obviously improve blood coagulation ability and obviously mitigate inflammatory reaction of MODS rats.