TRPV4对HeLa细胞增殖的作用

The effect of TRPV4 regulation on HeLa cells proliferation in vitro

目的观察瞬时感受器电位离子通道香草素受体4(TRPV4)与宫颈癌HeLa细胞增殖的关系。方法采用宫颈癌HeLa细胞系,用Western blot技术检测HeLa细胞中TRPV4的表达量;并分别用TR-PV4阻滞剂RN1734干预体外培养HeLa细胞,通过细胞计数法、LDH检测及流式细胞技术观察TRPV4通道调控对HeLa增殖和细胞周期进展的影响。结果与对照组W12细胞系相比,宫颈癌HeLa细胞系中TRPV4呈高表达。TRPV4阻滞剂干预48和72h后,HeLa细胞增殖较未干预组降低(P<0.05);而TRPV4阻滞剂干预24h和48h时,处于S期细胞的百分率明显低于未干预组;处于G0/G1期细胞的百分率则明显高于未干预组(P<0.05)。结论 TRPV4抑制可以阻滞体外培养宫颈癌HeLa细胞增殖及细胞周期进展。

Objective To observe the effect of transient receptor potential vanilloid 4 regulation on HeLa cells proliferation in vitro.Methods Expressed TRPV4 by HeLa and W12 cells were measured by western blot.Then HeLa cells were randomly divided into two groups：control group and RN1734 group.At different time points （0 h,12 h,24 h,48 h and 72 h）,the changes of HeLa proliferation and cell cycle progression were detected by cell number counted assay,LDH release assay and flow cytometry.Results Compared with W12 cells,the expression of TRPV4 was increased in HeLa cells.The proliferation of HeLa cells was blocked by RN1734 （P ＜ 0.05）.At the mean time,the percentage of HeLa cells in S phase in RN1734 groups were significantly less （P ＜0.05） than control groups while that in G0/G1 phase were increased at these time points.Conclusions Transient receptor potential vanilloid 4 blocker RN1734 can remarkably inhibit HeLa cells prcliferationin vitro.Our results suggested that the transient receptor potential vanilloid 4 were involved in HeLa cells proliferation.