摘要
用化学修饰法合成了一个新的具有GPX活性的模拟物-硒化透明质酸(SeHA),其GPX活力可达276.88 U/μmol。用红外光谱和核磁共振光谱对模拟酶的结构进行研究,证明硒的修饰位点位于透明质酸的N-乙酰氨基葡萄糖的-CH2OH。为进一步研究SeHA的抗氧化活性,建立了Fe2+/Vc诱导的牛心线粒体损伤体系,对SeHA的体外生物活性进行研究。结果显示SeHA能够有效抑制损伤线粒体的膜膨胀、脂质过氧化终产物丙二醛(MDA)的生成及线粒体细胞色素氧化酶(CCO)活力的降低;同时在与另一种GPX模拟物-PZ51的比较中SeHA也表现出了更为明显的体外抗氧化活性。
Basing on the framework of hyaluronic acid（ HA） and method of chemical modification,a novel selenide hyaluronic acid（ SeHA） was synthesized to be functioned as a mimic of glutathione peroxidase（ GPX） with activity of 276. 88 U / μmol. The structure of SeHA was characterized by means of IR and NMR with the conclusion that the target SeH was located at —CH2OH of the N—acetyl- D- glucosamine. Antioxidant activity was studied by using ferrous sulfate / ascorbate-induced mitochondria damage model system,The result demonstrated that SeHA could obviously inhibited the swelling of mitochondria,reduced the accumulation of MDA、preserved the activity of cytochrome-coxidase in the mitochondria damage model system. Compared with PZ51,the GPX activity of SeHA was more conspicuous.
出处
《科学技术与工程》
北大核心
2014年第15期148-152,共5页
Science Technology and Engineering
基金
沈阳医学院科技基金项目(20122035)资助
