胃肠道肿瘤中EphrinB2和淋巴管密度的表达及临床意义

Expression and their clinical significance of EphrinB2 and lymphatic vessel density in gastrointestinal carcinomas

目的探讨胃肠道肿瘤中EphrinB2的表达与淋巴管密度、淋巴结转移及临床病理参数的关系。方法采用免疫组织化学、蛋白质印迹两种方法检测EphrinB2在45例胃腺癌、57例大肠腺癌和正常胃大肠黏膜组织(各15例)中的表达。检测D2-40标记的淋巴管并计数淋巴管密度(LVD)。结果 (1)Eph-rinB2阳性反应主要定位于肿瘤细胞胞质内;D2-40标记的LVD为棕黄色管腔样结构,阳性反应主要位于淋巴内皮的胞膜上。(2)免疫组织化学:EphrinB2在肿瘤组织中阳性表达率明显高于正常组织的阳性表达率(胃82.2%vs.26.7%,大肠75.4%vs.33.3%,P均<0.05);肿瘤组织LVD显著高于正常组织(P均<0.05)。蛋白质印迹:胃和大肠肿瘤组织的表达分别为2.30±0.86和1.89±0.89,高于正常组织的表达0.90±0.41和0.87±0.56(P均<0.05)。(3)EphrinB2的表达与肿瘤分化程度、淋巴结转移及临床分期有密切关系(P<0.05),而与年龄、性别、肿瘤大小无明显相关性(P>0.05)。肿瘤组织中LVD与肿瘤分化程度、淋巴结转移、远处转移及临床分期相关(P<0.05),Spearman秩相关分析发现EphrinB2的表达与LVD呈正相关性。结论 EphrinB2在肿瘤中高表达,与胃肠道肿瘤的转移和进展密切相关,并与LVD呈正相关性,Eph-rinB2可能诱导LVD的形成参与淋巴结转移的调控。

Objective To study the expression and clinical signifance of EphrinB2 and lymphatic vessel density （LVD） in gastrointestinal carcinomas and normal tissue.Methods To examine the expression of EphrinB2 by using immunohistochemistry and western blot technique in 45 samples of gastric carcinoma,57 samples of colorectal carcinoma,15 samples of gastric normal tissue and 15 samples of colorectal normal tissue.LVD was marked by D2-40with immunohistochemistry.Results （1） Staining of EphrinB2 was mainly in cytoplasm of the carcinoma cell.The brown and fielemot lumen was observed by the means of D2-40 in normal and carcinoma tissue.（2）IHC：The positive rate of EphrinB2 in carcinomas was higher than that in normal tissue （stomach 82.2% vs.26.7%,large intestine 75.4% vs.33.3%,P 〈 0.05）,LVD in carcinomas was significantly higher than that in the normal tissue （P 〈0.05）.Western blot：Protein level in carcinomas was 2.30 ± 0.86 and 1.89 ± 0.89 respectively,which was also higher than that in normal （0.90 ± 0.41 and 0.87 ± 0.56,P 〈 0.05）.（3） The high expression of EphrinB2 in gastrointestinal carcinoma was closely correlated with histological type,metastasis of lymph nodes and the surgicalpathological stages（P 〈0.05）.LVD by the means of D2-40 was correlated with the histological type,metastasis of lymph nodes,distant metastasis and surgical-pathological stages （P 〈 0.05）.Spearman analysis showed that the expression of EphrinB2 was positively correlated to the LVD of the tumor（P 〈0.05）.Conclusions The expression of EphrinB2 in gastrointestinal carcinomas were significantly high and they may play important roles in the process of metastasis and development in gastrointestinal carcinoma,and EphrinB2 also has positively correlated to the LVD.EphrinB2 has effects on the lymphangiogenesis of gastrointestinal carcinoma and regulating lymphatic metastasis.