α-亚麻酸对实验性糖尿病动物内皮功能保护作用的研究

Effects of alpha-linolenic acid on protection of endothelial cells in experimental animal model of diabetes

目的通过对实验性糖尿病动物模型外周血中内皮细胞功能相关标志物的测定,推测α-亚麻酸在保护血管内皮功能中的作用。方法制备实验性糖尿病动物模型,分为3组进行喂养:(1)正常对照组(Control组,n=12);(2)糖尿病对照组(DM组,n=14);(3)ALA处理组(DM+ALA组,n=14)。喂养4周后,测定外周血中NO、内皮素(ET)-1、可溶性P选择素(sP-selectin)和可溶性细胞间黏附分子(sICAM)-1的含量,并应用离体灌流法评价ALA对血管舒张功能的影响。结果 (1)DM组血清中NO水平明显低于Con-trol组(P<0.01),DM+ALA组血清中NO水平明显高于DM组(P<0.01);(2)DM组血清中ET-1水平明显高于Control组(P<0.01),DM+ALA组血清中ET-1水平明显低于DM组(P<0.01);(3)DM组血清中sP-selectin和sICAM-1水平明显高于Control组(P<0.01),DM+ALA组血清中sP-selectin和sICAM-1水平明显低于DM组(P<0.01);(4)DM+ALA组的大鼠降主动脉对ACh的舒张反应较DM组明显增强(P<0.01)。结论应用α-亚麻酸干预后,实验性糖尿病动物外周血中内皮损伤标志物的水平下降,而NO的水平升高,说明α-亚麻酸对内皮功能具有一定的保护作用。

Objective To determine whether diet rich in ALA may attenuate endothelial dysfunction associated with diabetes in experimental animal model through evaluating the serum level of NO, ET-1, sP-selectin and sICAM-1. Methods The experimental animal model of diabetes were divided into three groups ： control group, diabetes group（ DM group）and diabetes group fed by diet rich in ALA （DM ＋ ALA group）. The animal model were sacrificed at 4 weeks later. The serum levels of NO, ET-I, sP-seleetin and slCAM-I were evaluated. The affeetion of ALA in vasodliation was evaluated by isolated perfusion method. Results （ 1 ） The serum level of NO in DM group was significantly lower than control group （ P 〈 0. 01 ）, and the serum level of NO in DM ＋ ALA group was significantly higher than DM group（P 〈 0. 01 ） ; （2）The serum level of ET-1 in DM group was significantly higher than control group（ P 〈 0. 01 ）, and the serum level of ET-1 in DM ＋ ALA group was significantly lower than DM group （P 〈 0. 01 ） ;（3 ）The serum level of sP-selectin and slCAM-1 in DM group was significantly higher than control group （ P 〈 0. 01 ）, and the serum level of sP-selectin and slCAM-1 in DM ＋ ALA group was significantly lower than DM group（P 〈 0.01）; （4）The relaxation response to ACh in rat descending aortic in group DM ＋ ALA was significantly enhanced compared with DM group. Conclusions Treatment with ALA significantly decreased the levels of soluble cellular adhesion molecules （slCAM-1, P-seleetin）and ET-1, and restored NO production in diabetic animals. These results demonstrate that diet rich in ALA has the potential to attenuate endothelial dysfunction.