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两株副溶血弧菌标准株的肠毒性及细胞毒性和溶血活性比较 被引量：2

Comparison of enterotoxicity,cytotoxicity and hemolytic activity between two pilot vibrio parahaemolyticus strains
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摘要 目的评价两株副溶血弧菌标准株在肠毒性、细胞毒性和溶血活性上的毒力差异。方法通过兔回肠袢实验、Raw264细胞毒性实验和兔红细胞溶血实验比较临床分离株RIMD2210633(tdh+、T3SS1+、T3SS2α+、T3SS2β-、trh-)和环境分离株S251(tdh-、T3SS1+、T3SS2α-、T3SS2β-、trh-)的毒力表型差异。结果 S251株产生的肠积液量、对Raw264细胞的细胞毒性均弱于RIMD2210633株[(12.37±1.07)mlvs.(1.50±1.50)ml;(35.69±3.07)%vs.(14.07±0.91)%],且有统计学差异(P<0.05),另外S251株对兔红细胞的相对溶血活性明显弱于RIMD2210633株,且具有统计学差异(P<0.01),这与基因预测结果相符。结论 S251株的肠毒性、Raw264细胞毒性和溶血活性均弱于RIMD2210633株,该两株菌可作为后续副溶血弧菌致病机制研究的模式菌株。 Objective To assess the differences in the enterotoxicity,cytotoxicity and hemolysis activity between two pilot strains of Vibrio parahaemolyticus.Methods The virulence phenotypes of a clinical strain RIMD2210633（tdh ＋,T3SS1 ＋,T3SS2α ＋,T3SS2β-,and trh-） and a environment strain S251 （tdh-,T3SS1 ＋,T3SS2α-,T3SS2β-,and trh-）were compared by the rabbit ideal loop,cytotoxicity,and hemolysis assays.Results The fluid accumulation in rabbit intestines and cytotoxicity against Raw264 cells detected for S251 strain were significantly weaker than those for RIMD2210633 strain,（12.37 ± 1.07） ml vs.（1.50 ± 1.50） ml and（35.69 ±3.07）％ vs.（14.07 ±0.91）％ respectively（P ＜0.05）.Additionally,the hemolytic activity against rabbit red blood cells for S251 strain were clearly weaker than that for RIMD2210633 strain（P ＜ 0.01）.These results were consistent with the virulence gene profiles observed for these two strains.Conclusions RIMD2210633 strain was significantly more toxic than S251 strain.These two strains can be used as the pilot ones for the follow-up Vibrio parahaemolyticus pathogenesis studies.
作者 马立芝 郭李平 王立祥 王凤林 刘亚华 赵志兵 张广州 周冬生 邱业峰
机构地区 武警总医院急救医学中心 军事医学科学院实验动物中心 微生物流行病研究所 病原微生物生物安全国家重点实验室
出处 《中华临床医师杂志（电子版）》 CAS 2013年第6期154-156,共3页 Chinese Journal of Clinicians(Electronic Edition)
基金 国家自然科学基金(31170127) 国家重点基础研究发展计划"973"项目(2009CB522604)
关键词 弧菌 副溶血性 肠毒性 细胞毒性 溶血活性 Vibrio parahaemolyticus Enterotoxicity Cytotoxicity Hemolytic activity
分类号 R378 [医药卫生—病原生物学]
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参考文献11

  • 1郭李平,徐镔蕊,杜德燕,姜汉雯,邱业峰,周冬生.副溶血弧菌毒力评价小鼠模型的建立与应用[J].中国实验动物学报,2012,20(4):31-34. 被引量：5
  • 2Bhattacharjee, R. N,Park, K.S,Okada, K,Kumagai, Y,Uematsu, S,Takeuchi, O,Akira, S,Iida, T,Honda, T.Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus[].Biochemical and Biophysical Research Communications.2005
  • 3Kodama T,Rokuda M,Park KS,et al.Identification and characteriza-tion of VopT,a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system2[].Cell Mi-crobiol.2007
  • 4Iida T,Yamamoto K.Cloning and expression of two genes encoding highly homologous hemolysins from a Kanagawa-phenomenonpositive Vibrio parahaemolyticus T4750 strain[].Gene.1990
  • 5Nishibuchi M,Fasano A,Russell RG,et al.Enterotoxigenicity of Vib-rio parahaemolyticus with and without genes encoding thermostable di-rect hemolysin[].Infection and Immunity.1992
  • 6Hiyoshi H,Kodama T,Iida T,et al.Contribution of Vibrio parahaemo-lyticus virulence factors to cytotoxicity,enterotoxicity,and lethality in mice[].Infection and Immunity.2010
  • 7Hsieh YC,Liang SM,Tsai WL,Chen YH,Liu TY, et al.Study of capsular polysaccharide from Vibrio parahaemolyticus[].Infection and Immunity.2003
  • 8Xiao X,Yan YF,Zhou DS,et al.A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs)and variable-number tandem repeats (VNTRs)[].International Journal of Food Microbiology.2011
  • 9Makino K,Oshima K,Kurokawa K,et al.Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae[].LancetThe.2003
  • 10Taniguchi H,Hirano H,Kubomura S,et al.Comparison of the nucleotide sequence of the genes for the thermostable direct hemolysin and the thermotabile hemolysin from Vibrio parahaemolyticus[].Microbial Pathogenesis.1986

二级参考文献11

  • 1Danie|s, G. R, L. Mackinnon. Vibrio parahaemolyticus infections in the United States, 1973 - 1998 [ J]. J Infect Dis, 2000, 181:1661 - 1666.
  • 2Hiyoshi H, Kodama T, lida T. Contribution of Vibrio parahaemo- lyticus virulence fators to cytotoxicity, enterotoxicity, and lethali- ty in mice [J]. Infect Immun, 2010, 78(4) :1772-1780.
  • 3Okuda J. Emergence of a unique 03 : K6 clone of Vibrio parah- aemolyticus in Calcutta, India, and isolation of strains from the same elonal group from SoutheastAslan travelers arriving in Japan [J]. JClin Mierobiol, 1997, 35(12): 3150-3155.
  • 4肖潇.一种基于LVPC与VNTR副溶血弧茵基因分型新方法[D].北京:军事医学科学院微生物流行病学研究所,2011.
  • 5Miyamoto Y, Kato T, Obara Y, et al. In vitro hemolytic charac- teristic of Vibrlo parahaemolyticus: its close correlation with hu- man pathogenicity [ J ]. J Bacteriol, 1969, 100 : 1147 - 1149.
  • 6Kim YB, Okuda J, Matsumoto C, et al. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene [J]. C|in MicrobioI, 1999, 37(4) : 1173 -1177.
  • 7Okura M, Osawa R, Iguchi A, et al. PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-spe- cific primer pair [ J]. Microbiol Immunol, 2004, 48 (|0) : 787 - 790.
  • 8Makio K,.Oshima K, Kurokawa K, et al, Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V cholerae [ J ], Lancet, 2003, 361:743-749.
  • 9Xiao X, Yah YF, Zhou DS, et al. A novel genotyping scheme for Vibrio parahaemolytlcus with combined use of large variably- presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs) [J]. lnt J Food Mierobiol, 2011, 149(2): 143 - 151.
  • 10Tada J, Ohashi T, Nishimura N, et al. Detection of the thermo- stable direct hemolysin gene (tdh) and the thermostable direct hemolysln-related hemolysin gene (trh) of Vibrio parahaemolytic- us by polymerase chain reaction [ J]. Mol Cell Probes, 1992, 6 (6): 477-487.

共引文献4

同被引文献22

  • 1Daniels NA, MacKinnon L, Bishop R, et al. Vibrio parahaemolyticus Infections in the United States, 1973-1998[J]. J Infect Dis,2000,181(5):1661-1666.
  • 2Shimohata T, Takahashi A. Diarrhea induced by infection of Vibrio parahaemolyticus[J].J Med Invest,2010,57(3/4):179-182.
  • 3Okuda J, Ishibashi M, Hayakawa E, et al. Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India, and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan[J]. J Clin Microbiol,1997,5(12):3150-3155.
  • 4Yeung PS, Boor KJ. Epidemiology, pathogenesis, and prevention of foodborne Vibrio parahaemolyticus infections[J]. Foodborne Pathog Dis,2004,1(2):74-88.
  • 5Broberg CA, Calder TJ, Orth K. Vibrio parahaemolyticus cell biology and pathogenicity determinants[J]. Microbes Infect,2011,13(12/13):992-1001.
  • 6Haines-Menges B, Whitaker WB, Boyd EF. Alternative sigma factor RpoE is important for Vibrio parahaemolyticus cell envelope stress response and intestinal colonization[J]. Infect Immun,2014,82(9):3667-3677.
  • 7Liu ZY, Wang YN, Liu SY, et al.Vibrio cholerae represses polysaccharide synthesis to promote motility in mucosa[J]. Infect Immun,2015,83(3):1114-1121.
  • 8Tamayo R, Patimalla B, Camilli A. Growth in a biofilm induces a hyperinfectious phenotype inVibrio cholerae[J]. Infect Immun,2010,78(8):3560-3569.
  • 9Whitaker WB, Parent MA, Boyd A, et al. The Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model[J]. Infect Immun,2012,80(5):1834-1845.
  • 10Nishibuchi M, Fasano A, Russell RG, et al. Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin[J]. Infect Immun,1992,60(9):3539-3545.

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中华临床医师杂志（电子版）
2013年 第6期

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