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白癜风患者外周血IL-27、IL-35蛋白表达及EBI3 mRNA表达的研究

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摘要 目的检测不同病期白癜风患者外周血IL-27、IL-35蛋白及其共同亚基EBI3 mRNA(Epstein-Barr virus-induced gene 3,EBI3)的表达。方法采用竞争性免疫抑制ELISA法检测白癜风患者不同病期、不同分型及健康对照者外周血血清IL-27、IL-35表达水平,采用SYBR Green Ⅰ实时荧光定量RT-PCR方法检测各期外周血单个核细胞(PBMC)中EBI3mRNA的表达水平。结果进展期、稳定期白癜风患者血清IL-27[(184.11±50.70)pg/ml、(167.74±39.84)pg/ml]和IL-35[(348.69±36.26)pg/ml、(316.01±53.68)pg/ml]表达均显著高于健康对照组[(138.66±33.93)pg/ml和(292.44±36.26)pg/ml],差异均有统计学意义(IL-27:P=0.000、P=0.003;IL-35:P=0.000、P=0.005);外周血单个核细胞EBI3mRNA在白癜风患者进展期、稳定期表达量较健康对照组分别高11.69、7.48倍;局限型、散发型、肢端型、泛发型白癜风患者血清IL-27[(174.57±43.15)pg/ml、(174.95±53.73)pg/ml、(166.30±27.40)pg/ml、(194.38±59.73)pg/ml]和IL-35[(322.72±65.18)pg/ml、(337.41±50.82)pg/ml、(328.04±33.42)pg/ml、(345.57±79.05)pg/ml]表达均显著高于健康对照组,各型组间比较无统计学差异。结论 IL-27、IL-35、EBI3可能参与白癜风发病。
出处 《中华临床医师杂志(电子版)》 CAS 2013年第6期179-180,共2页 Chinese Journal of Clinicians(Electronic Edition)
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参考文献8

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