补肾活血中药对大鼠慢性高眼压模型视神经BDNF改变的影响

Effects of traditional Chinese medicine of BuShenHuoXue on the expression of brain-derived neurotrophin factor onoptic nerve in rat model of chronic elevated intraocular pressure

目的：观察补肾活血中药对大鼠慢性高眼压（elevated intraocular pressure ，EIOP）模型视神经（optic nerve ，ON）脑源性神经生长因子（brain-derived neurotrophin factor ，BDNF）的干预作用，探讨其作用机理。方法采用烙闭上巩膜静脉法，烙闭大鼠3支上巩膜静脉，建立大鼠慢性EIOP模型，随机分为3组：空白组，模型组，给药组。连续灌胃8周，并于8周末处死大鼠，观察补肾活血法对EIO P大鼠眼压、视神经的BDNF表达的影响。结果本实验采用的烙闭上巩膜静脉的造模方法使大鼠IOP明显升高（P＜0．01），在造模后8周（实验结束）IOP仍为造模前的2～3倍；BDNF表达总面积、平均光密度、积分光密度空白组（199882．9±24012．79μm2、334．3±52．58、41453．0±1646．85）与模型组（148219．4±17978．48μm2、241．2±49．17、37391．7±2533．45）比较均有统计学意义（ P＜0．01）；模型组与给药组（148219．4±17978．48μm2、301．2±36．44、40439．1±2000．05）比较亦均有统计学意义（ P＜0．01）。结论慢性高眼压大鼠视神经BDNF表达降低，补肾活血中药有助于恢复EIOP大鼠视神经BDNF表达。

Objective To observe the effect traditional Chinese medicine（TCM） of BuShenHuoXue on the expression of brain-derived neurotrophin factor （BDNF ） on optic nerve in the rat model of chronic elevated intraocular pressure （EIOP） ,and preliminarily explore its mechanism .Methods the rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral vessels ,then randomly divided into 3 groups ：control group ,model group and treatment group .After given TCM of BuShenHuoXue and normal saline for 8 weeks ,killed the rats and taken out optic nerve .The effect of TCM of BuShenHuoXue on the EIOP rat model’s intraocular pressure（IOP） and the effection of the expression on BDNF in optic nerve was observed .Results Unilaterally cauterizing episcleral vessels increased IOP of the rat modle obviously （P〈0 . 01） .At the end of the experiment ,the level of IOP was 2~3 times than Before modeling ;BDNF in optic nerve of total area ,mean optical density and integrated optical density in model group （148219 .4 ± 17978 . 48μm2 ,241 .2 ± 49 .17 ,37391 .7 ± 2533 .45）was statistically significant compared with normal group （199882 . 9 ± 24012 .79μm2 ,334 .3 ± 52 .58 ,41453 .0 ± 1646 .85 ,all P〈 0 .01 ） ,there was statistically difference in BDNF of total area ,mean optical density and integrated optical density between model group and treatment group （180755 .6 ± 17206 .34μm2 ,301 .2 ± 36 .44 ,40439 .1 ± 2000 .05 ,all P〈 0 .05） .Conclusion The expression of BDNF decreased in the rat model of chronic EIOP ,TCM of BuShenHuoXue can improved the expression of BDNF on optic nerve in the EIOP rat model .