树突状细胞/细胞因子诱导的杀伤细胞人体内逆转肺癌多药耐药的临床研究

Clinical study of DC/CIK cells reverse multidrug resistance on lung cancer in the body

目的探讨树突状细胞/细胞因子诱导的杀伤细胞(DC/CIK)是否具有人体内逆转肺癌多药耐药(MDR)的作用。方法 30例晚期非小细胞肺癌患者接受2个疗程DC/CIK细胞输注。输注前和完成2个疗程细胞输注后,流式细胞术测定P-糖蛋白(P-gp)和多药耐药相关蛋白(MRP)阳性细胞百分率以及平均荧光强度。结果 30例晚期非小细胞肺癌患者全部检测出P-gp和MRP阳性细胞。DC/CIK细胞输注前,P-gp阳性细胞百分率为(38.67±8.76)%,平均荧光强度2.18±0.34;MRP阳性细胞百分率为(1.14±0.49)%,平均荧光强度为2.14±0.437。DC/CIK细胞输注后,P-gp阳性细胞百分率为(34.96±6.9)%,平均荧光强度2.07±0.42;MRP阳性细胞百分率为(1.0±0.53)%,平均荧光强度为2.05±0.42。2个周期DC/CIK细胞输注前后,P-gp阳性细胞百分率的差异有统计学意义(t=5.02,P<0.001),平均荧光强度的差异有统计学意义(t=2.18,P<0.05);MRP阳性细胞百分率的差异有统计学意义(t=2.35,P<0.05),其平均荧光强度的差异有统计学意义(t=2.16,P<0.05)。结论 DC/CIK细胞可以在人体内逆转肺癌MDR。

Objective To investigate whether DC/CIK（Dendritic cells/Cytokine induced killer cells） cell can reversal of multidrug resistance （MDR） on lung cancer in the body. Methods 30 cases of patients with advanced non-small cell lung cancer received 2 cycles of DC/CIK cell infusion. Before and after 2 cycles of DC/CIK cells infusion, the percentage of P-gp-positive cells and MRP-positive cells were determined by flow cytometry. Mean fluorescence intensity was determined by flow cytometry. Results 30 cases of patients with advanced non-small cell lung cancer were all detected P-gp-positive cells and MRP-positive cells. Before DC/CIK cells infusion, the percentage of P-gp-positive cells was 38.67±8.76, the fluorescence intensity of P-gp was 2.18 ±0.34; the percentage of MRP- positive cell was 1.14±0.49, the fluorescence intensity of MRP was 2.14±0.437. After DC/CIK cells infusion, the percentage of P-gp-positive cells was 34.96±6.9, the fluorescence intensity of P-gp was 2.07±0.42;the percentage of MRP-positive cell was 1.0±0.53, the fluorescence intensity of MRP was 2.05±0.42. The difference of the percentage of P-gp-positive cells was statistically significant（P〈0.001） before and after 2 cycles of DC/CIK cells infusion. The fluorescence intensity of P-gp also had significant difference（P〈0.05）.The difference of the percentage of MRP-positive cells was statistically significant（P〈0.05） before and after 2 cycles of DC/CIK cells infusion. The fluorescence intensity of MRP had significant difference（P〈0.05）. Conclusion DC/CIK cells can reverse the multidrug resistance on lung cancer in the body.