Omi/HtrA2及Caspase-8在结直肠癌组织中的表达及其意义

Expression and significance of Omi /HtrA2 and Caspase-8 protein in colorectal carcinoma

目的研究结直肠癌中Omi/HtrA2(丝氨酸蛋白酶)和Caspase-8的表达与预后的关系及其临床意义。方法用免疫组化检测96例结直肠癌组织,39例癌旁组织中Omi/HtrA2及Caspase-8的表达,并分析其与结直肠癌的临床病理特征及预后的关系。结果癌组织中Omi/HtrA2的强表达率72.9%(70/96)明显高于癌旁组织中的强表达率33.3%(13/39,P<0.05)。癌组织中Caspase-8的强表达率77.0%(74/96)明显高于癌旁组织中的强表达率33.3%(13/39,P<0.05)。结直肠癌组织中Omi/HtrA2的表达与病理分化程度、淋巴结转移、TNM分期相关(P<0.05),与年龄、性别、浸润深度、辅助化疗及肿瘤大小无关(P>0.05);Caspase-8的表达与淋巴结转相关(P<0.05),与年龄、性别、肿瘤大小、病理分化程度、浸润深度、辅助化疗及TNM分期无关(P>0.05)。Omi/HtrA2及Caspase-8在癌组织中的表达进行相关性分析显示二者没有相关性,均与预后不相关(P>0.05)。结论结直肠癌中Omi/HtrA2及Caspase-8均强表达,二者无相关性;Omi/HtrA2的表达与病理分化程度、淋巴结转移及TNM分期有关;Caspase-8的表达与淋巴结转移有关;Omi/HtrA2和Caspase-8均不是影响其预后的因素。

Objective To investigate the expression of serine protease Omi/HtrA2 and Caspase-8 protein in colorectal carcinoma and its relationship with prognosis. Methods The expression of Omi /HtrA2 and Caspase-8 protein were detected in colorectal carcinoma（n=96） and adjacent non-carcinoma tissues（n=39）by immunohistochemical method, and its association with clinicopathologic features and prognosis were analyzed. Results The high expression rates of Omi/HtrA2 and caspase-8 in colorectal carcinoma were 72.9%（70/96） and 77.0%（74/96）, with 33.3%（13/39） and 33.3%（13/39） of adjacent non-carcinoma tissues respectively. Significant differences between the groups（P〈0.05）can be detected. The expression of Omi/HtrA2 was significantly associated with tumor histological differentiation, lymph node metastasis and TNM stage（P〈0.05）, while no significant difference with age, sex, invasion depth and tumor size. The expression of caspase-8 was significantly associated with lymph node metastasis （P〈0.05）, however, no significant difference was observed with age, sex, tumor histological differentiation, invasion depth, TNM stage, chemotherapy and tumor size. For Omi/HtrA2 and Caspase-8 expression in cancer tissues, no correlation was observed（P〉0.05）. Neither Omi/HtrA2 nor Caspase-8 was prognostic factor. Conclusions Omi/HtrA2 and caspase-8 are highly expressed in colorectal cancer tissues. The expression of Omi/HtrA2 is significantly related to the degree of carcinoma differentiation, clinical pathological stage and lymph node metastasis; while the expression of caspase-8 is only significantly related to lymph node metastasis. Omi/HtrA2 as well as caspase-8 has no impact on the survival of colorectal cancer patients.