蛋白质组学在病毒致病机理研究中的应用

The Application of Proteomics in Viral Pathogenesis Research

近年来,蛋白质组学技术成为医学研究的热点。蛋白质组学是高通量的分析正常及病理条件下机体、组织、细胞或亚细胞成分中的全部蛋白质。对不同空间、不同时间上动态变化的蛋白质组的整体进行比较,分析不同蛋白质组之间在表达数量、表达水平和修饰状态上的差异。蛋白质组学分析作为对生物代谢调控分析的技术手段,以病毒为研究的对象和工具,该技术的研究主要集中在新蛋白的发现、致病机理、疫苗的研制及耐药机制等方面。本文主要概述了蛋白质组学在一些动物传染病病毒致病方面研究和应用,分析了蛋白质组学技术对蛋白功能研究存在的问题和未来发展趋势,以便使研究者了解该技术使用的现状,提供理论参考。

In recent years, the proteomics technology is applied to study on medical research. Proteomics is analysis of normal and pathological conditions organism, tissue, cell or subcellular component of all proteins in a high throughput, through comprehensive comparison of dynamic change on different spatial and time proteomic, analysis the different between proteome expression quantity, differences of expression level and modification status. Proteomic analysis technology of biological metabolic control is used virus as research tool. And the technology research mainly focused on the new protein findings, pathogenesis, vaccine development and resistance mechanism, etc. Research advances in proteomics were summarized focused on some animal infectious viral pathogenesis research and application. The problems relating to research and tendency in future of proteomics technologies were also analyzed in order to better understand the technologies, furthermore guide their theoretical reference.