负压封闭引流对兔颅骨外露创面愈合效果的实验研究

Effects of Vacuum Sealing Drainage on the Treatment of Cranial Bone Exposure in Rabbits

目的:探讨负压封闭引流技术(VSD)对兔颅骨外露缺损创面愈合的治疗效果。方法:选取成年新西兰大白兔76只,平均分为四组并建立兔颅骨外露实验模型。其中,A组(19只):于兔颅骨上方制作直径为2.0cm的圆形创面,保留骨膜,采用-120mmHg负压引流和常规换药治疗;B组(19只):实验动物处理同A组,仅采用常规换药治疗;C组(19只):在兔颅骨上制作直径2.0cm的圆形创面,剔除骨膜,治疗方法同A组;D组(19只):实验动物处理同C组,治疗方法同B组。每组各抽取10只,观察创面愈合率和创面愈合时间;其余9只分别在第7天、10天、20天、30天进行取材检测,分析疗效机制。结果:A组创面愈合时间为19.40±1.65天,B组为24.00±2.31天;C组为25.40±4.43天,D组为30.00±5.50天。运用VSD治疗和常规治疗创面愈合时间比较有统计学意义(P<0.05)。结论:VSD治疗兔骨外露缺损创面能有效缩短创面愈合时间,促进血管再生,胶原蛋白合成。

Objective： To investigate the curing effect of vacuum sealing drainage（VSD） on the cranial bone exposure in rabbits. Methods： 76 healthy white New Zealand Rabbits were selected and divided into four groups with 19 in each one for the establishment of the experimental animal models. A cranium wound of in diameter with（group A and B） or without periosteum（group C and D） were established in the rabbits, which were then treated by the regular medication with（group A and C） or without the combination of-120mmHg vacuum sealing drainage（VSD）（group B and D）. Ten rabbits of each group were monitored for the wound healing rate and time and the others were used on the day 7, 10, 20, and or the theraperutic mechanism studies. Results： The healing time was 19.40± 1.65 days in group A, 24.00±2.31 days in group B, 25.40±4.43 days in group C and 30.00±5.50 days in group D. The VSD-treated groups showed significant difference compared with control groups（P〈0.05）. Conclusions： VSD treatment can effectively shorten healing time and accelerate revascularization and collagen protein synthesis in rabbit cranial bone exposure wound.