脂联素rs＋16861194多态性在2型糖尿病中的研究

Research of Adiponectin rs＋16861194 polymorphism in type 2 diabetes

目的：（1）明确2型糖尿病患者和健康人群的脂联素rs＋16861194（A/G）位点的三种基因型分布是否有差异；（2）探讨脂联素rs＋16861194的基因多态性与T2DM及健康人群部分生化指标水平的关系。方法：选取158例2型糖尿病患者为实验组,132例健康体检人群为对照组。分别对实验组和对照组进行联素基因rs＋16861194基因型进行检测；同时检测空腹葡萄糖,甘油三酯（TG），高密度脂蛋白-胆固醇（HDL），低密度脂蛋白-胆固醇（LDL）；采用化学发光法测定空腹胰岛素,并计算胰岛素抵抗指数。结果：①T2DM组和CON组脂联素rs＋16861194位点的基因型分布分别为：AA（70.00%）、AG（24.37%）、GG（5.63%）和AA（74.24%）、AG（24.24%）、GG（1.52%）；②以脂联素rs＋16861194位点的基因型分组，在T2DM组和CON组内，各基因型之间的FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH水平均无统计学差异（P〉0.05）。③在T2DM组与CON组之间比较，T2DM组AA型与CON组AA型的FBG，FIN，IRI，TG，HDL差异有统计学意义（P〈0.05）；CHOL，LDL差异无统计学意义（P〉0.05）。T2DM组AG＋GG型与CON组AG＋GG型的FBG，IRI，TG，HDL差异有统计学意义（P〈0.05）；FIN，CHOL，LOL差异无统计学意义（P〉0.05）。结论：（1）脂联素rs＋16861194（A/G）位点的三种基因型分布无显著性差异。（2）脂联素rs＋16861194的基因型在2型糖尿病人群内或健康人群内对FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH水平无影响。

Objective：（1） To explore the difference of three kinds of adiponectin rs ＋16861194 （A / G ） genotype distribution in the type 2 diabetes patients and healthy people ; （2） To investigate the relationship of adiponectin rs＋16861194 gene polymorphism with some biochemical indicators from T2DM and control.Methods：Selected 158 cases of type 2 diabetes patients as the experimental group, 132 cases of healthy people as the control group. The genotypes of adiponectin rs-16861194 in experimental group and the control group were detected; At the same time, fasting plasma glucose, triglyceride （TG）, high density lipoprotein cholesterol （HDL）, low density lipoprotein cholesterol （LDL） were measured seperately. Fasting insulin was measured by chemiluminescence and insulin resistance index was calculated.Results：①T2DM group and CON group adiponectin rs ＋16861194 genotype distribution were： AA （70.00%）, AG （24.37%）, G （5.63%） and AA （74.24%）, AG （24.24%）, GG （1.52%）, the distribution showed no statistically significant difference （P〉 0.05）; ②Grouped by the genotype of adiponectin rs ＋16861194, the level of FBG, FIN, IRI, TG, CHOL, HDL-CH, LDL-CH showed no statistically significant difference （P〉 0.05） between the genotypes in T2DM and CON group. ③ Compraring between T2DM group and CON group,.the level of FBG, FIN, IRI, TG, HDL in the AA type of T2DM group and CON group had significant difference （P〈0.05）, while the level of CHOL and LDL showed no statistically significant difference （P〉 0.05） ; The level of FBG,IRI,TG,HDL in the AG＋GG type of T2DM group and CON group had significant difference （P〈0.05）, while the level of FIN,CHOL,LOL showed no statistically significant difference （P〉 0.05）.Conclusions：（1） Three kinds of locus genotype distribution of rs ＋16861194 （A / G） had no significant difference in Type 2 diabetes patients and healthy people.（2） The genotype of adiponectin rs＋16861194 had no effect on the level of FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH in type 2 diabetes patients and healthy people.