MMP-2、MMP-9及其抑制因子TIMP-2、TIMP-1联合检测对宫颈癌浸润转移潜能的预测价值

Evaluated value of invasion and metastasis of combined examination of MMP-2,MMP-9 and TIMP-2,TIMP-1 in cervical cancer

目的分析基质金属蛋白酶2(MMP-2)和MMP-9及其抑制因子2(TIMP-2)和TIMP-1联合检测对宫颈癌浸润转移潜能的预测价值。方法选取经病理证实的宫颈浸润癌患者112例(ICC组)、宫颈上皮内瘤样病变患者56例(CIN组);同时,选取正常宫颈组织标本30例为对照组。采用SABC法行免疫组织化学检测MMP-2、MMP-9、TIMP-2和TIMP-1的表达情况。比较3组相关因子阳性表达率的差异,并分析ICC组各因子阳性表达率与临床病理参数的关系。结果 ICC组MMP-2和MMP-9的阳性表达率显著高于CIN组和对照组,TIMP-2和TIMP-1的阳性表达率显著低于CIN组和对照组(P<0.05)。ICC组中,MMP-2、MMP-9、TIMP-2和TIMP-1的阳性表达率与国际妇产科联盟(FIGO)分期、组织学分级、分化程度、脉管浸润和淋巴结转移有关(P<0.05)。结论 MMP-2、MMP-9及其抑制因子TIMP-2、TIMP-1可能与宫颈癌的发生、发展有关,其可作为评估宫颈癌侵袭转移潜能的重要指标。

Objective To investigate the evaluated value of invasion and metastasis of combined examination of matrix metalloproteinase （MMP-2,MMP-9） and tissue inhibitor of matrix metalloproteinase （TIMP-2,TIMP-1） in cervical can- cer. Methods 112 cases of patients with early invasive cervical cancer （ICC） and 56 cases of patients with cervical in- traepithelial neoplasm （CIN） were selected,and 30 cases of normal cervical epithelium （NCE） were selected as control group. The expression of MMP-2,MMP-9,TIMP-2 and TIMP-1 were examined by SABC method of immunohistochemistry.Ex- pression rate of every factor in different groups and the factors related to clinical pathology of ICC group were analyzed. Rusults Compared with control group and CIN group,expression rate of MMP-2,MMP-9 in ICC group were higher, TIMP-2 and TIMP-1 in ICC group were lower （P〈0.05）.In ICC group,expression rate of MMP-2,MMP-9,TIMP-2, TIMP-1 were correlated with FIGO clinical stage,histological grading,differentiated degree,intravascular infiltration and lymph node metastasis （P〈0.05）. Conclusion MMP-2,MMP-9 and TIMP-2,TIMP-1 may play an important role in iva- sion and metastasis of cervical cancer.The expression can be used to estimate the metastasis potentiality.