高浓度胰岛素水平对阿司匹林抑制的血小板聚集率的影响

Effect of High Concentrations of Insulin Levels to Aspirin Inhibits Platelet Aggregation Rate

目的 研究高浓度的胰岛素是否影响阿司匹林抑制的血小板聚集率.方法 将制备的4组等体积的富含血小板血浆（PRP）分别加入0,30,100和300μU/ml的胰岛素,37℃的环境下孵育3 min,再将上述每一组的PRP分成等体积的4个组,分别加入0,75,150和300 μmol/L的阿司匹林,于37℃的环境下继续孵育30 min.用血小板聚集仪检测各组的血小板最大聚集率.结果 不同浓度胰岛素组之间有显著差异（F=4.056,P=0.008）.300 μU/ml胰岛素浓度的富含血小板血浆,在不同浓度阿司匹林0,75,150和300 μmol/L的孵育下,血小板最大聚集率分别为（87.62±6.23）％,（60.85±16.24）％,（55.28±16.48）％和（49.36±16.77）％,均高于相应对照组的血小板最大聚集率（83.82±8.35）％,（48.80±21.35）％,（45.04±21.44）％,（38.90±21.70）％和30 μU/ml胰岛素组的血小板最大聚集率（84.64±8.09）％,（48.13±19.27）％,（44.30±20.50）％和（35.30±16.81）％,其差异有统计学意义（P＜0.05）;而其他浓度的胰岛素组间比较,差异则无统计学显著性意义.结论 血小板暴露于高浓度的胰岛素（300 μU/ml）环境中,将会减弱阿司匹林对血小板的抗聚集效应.

Objective To examine if acute exposure to increased plasma insulin impaired the inhibitory effects of aspirin onplatelet aggregation rete. Methods The equivalent volume of platelet-rich plasma （PRP） prepared by hand was preincubatedwith insulin（0,30,100,300 μU/ml） in 37℃ water for 3 minutes,which was then stimulated with different concentrations ofaspirin （0,75,150,300μmol/L） for 30 minutes in water of 37℃. The platelet maximal aggregation each sample were ana-lyzed by platelet aggregation system. Results There were significant differences between groups with different concentra-tions of insulin （F=-4. 056,P=0. 008）. The inhibitory effects of 75,150 and 300 /μmol/I. aspirin differed in the presence of300/iU/ml vs. the absence of insulin （87.62±6.23）%,（60.85±16.24）%,（55.28±16.48）%vs（49.36±16.77）% , whereas they were not significant among other concentrationsof insulin. Conclusion In vitro exposure to high insulin （i. e. ,300 μU/ml） of platelets from healthy subjects reduces the an-tiaggregating action of aspirin.