毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响

Effect of Calycosin on Proliferation,Migration,and Invasion of Human Melanoma Cell A375 in vitro

目的：研究毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响.方法：以人黑色素瘤细胞A375为研究对象,MTT法检测不同浓度的毛蕊异黄酮对A375细胞活性的影响,划痕实验检测A375细胞的迁移,Transwell小室检测A375细胞的侵袭作用;qPCR检测A375细胞中基质金属蛋白酶2（MMP-2）及基质金属蛋白酶9（MMP-9）表达水平.结果：毛蕊异黄酮在6.25 ～ 100 μmol/L浓度内不能抑制A375细胞的增殖,划痕实验及Transwell检测结果显示毛蕊异黄酮可显著抑制A375细胞的迁移及侵袭（P＜0.01）,qPCR结果显示毛蕊异黄酮能抑制A375细胞MMP-2及MMP-9的表达.结论：毛蕊异黄酮在小于100 μmol/L浓度时抑制A375细胞的迁移及侵袭,其机制可能与抑制MMP-2及MMP-9表达有关.

Objective： To investigate the effect of calycosin on proliferation, migration, and invasion of human melanoma cell A375. Method： Human melanoma cell A375 was chosen as study suhject; The cell viability was determined by MTT assay after treated with calycosin, and the migration and in- vasion of A375 cells were measured by scratch test and transwell assay; qPCR was used to detect the expression levels of matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （ MMP-9 ） in A375 cells. Result： Calycosin could not inhibit A375 cell proliferation in the concentration range of 6.25 -100 μmol/L. Calycosin can inhibit A375 cell migration and invasion, and qPCR results confirmed that calycosin could inhibit the mRNA expression of MMP-2 and MMP-9. Conclusion： When the concentration is less than 100 μmol/L, calycosin can attenuate A375 cell migration and invasion, and the mechanism may relate to inhibiting the mRNA expression of MMP-2 and MMP-9.