氰戊菊酯对菲律宾蛤仔染毒后消化腺细胞的彗星实验分析

The analysis of digestive glands cells of Ruditapes philippinarum exposed to fenvalerate with comet assay method

为检测氰戊菊酯对菲律宾蛤仔染毒培育25 d后消化腺细胞DNA的损伤情况,将菲律宾蛤仔分为6组进行不同剂量染毒,采用彗星实验技术进行DNA损伤分析,并通过Comet Score 1.5分析软件对拖尾率、尾长、彗尾DNA相对含量、尾矩、Olive矩等DNA损伤指标进行统计。在对各指标分别建立一元回归方程的基础上,又通过SPSS软件进行判定分析,筛选影响作用更大的检测指标,建立了多参数的多元回归方程。结果显示,与空白对照组相比,菲律宾蛤仔各染毒组消化腺细胞DNA均有不同程度的损伤且各检测指标均有显著性差异,对照组受损细胞极少,拖尾率仅为8.06%±1.94%,彗尾DNA相对含量、尾矩和Olive矩都接近0,0.067 5-1.080 0 mg/L染毒实验组各指标值显著增加。研究表明,各检测指标与染毒浓度表现出良好的剂量效应关系,随着染毒浓度递增,各检测指标都呈规律性地增长趋势,具有高度的相关性,多项式回归方程和多元回归方程均有显著性的统计学意义,通过多元回归方程可以有效地推断氰戊菊酯染毒浓度和染毒时间。

In order to detect the DNA damage in digestive glands cells of Ruditapes philippinarum exposed to different concentrations of fenvalerate for 25 days respectively,R. philippinarum were divided into six groups and evaluated with comet assay method. The DNA damage parameters such as the comet rate,the tail length,the percentage of tail DNA,the tail moment and the olive moment were statistically analyzed by Comet Score 1. 5. On the basis of one-dimensional regression equation we selected greater influenced fly parameters after comparing with SPSS 16. 0 and constructed the multiple-dimensional regression equation. The results showed that DNA damages in digestive glands cells of all exposed groups were observed and the parameters of each exposed group were significantly different from the control group（ P 0. 01）. The DNA damage in digestive glands cells of control group was minimal. The tail rate of control group was 8. 06% ± 1. 94%,the percentage of tail DNA,tail moment and olive moment were nearly 0. The value of each parameter of all experimental groups with exposure concentration from 0. 067 5 mg / L to 1. 080 0 mg / L was significantly increased. Our research indicated that there was a dose-effect relationship between fenvalerate and DNA damage in digestive glands cells,which increased regularly with the exposure concentrations. All DNA damage parameters were highly relevant to the exposure concentrations. The one-dimensional regression equation（ P〈0. 05） and multiple-dimensional regression equation（ P〈 0. 02） had significant statistic meaning,which might be an effective way to estimate the exposure concentration and time of fenvalerate. Our research provided newmethods and clues to analyze the genotoxicity of aquatic species caused by the pollutant of pyrethroid pesticides in the coastal sea of China,which could be broadly applied.